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新型中药制剂肃毒星对乙型肝炎病毒体外复制的抑制作用研究
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新型中药制剂肃毒星对乙型肝炎病毒体外复制的抑制作用研究
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新型中药制剂肃毒星对乙型肝炎病毒体外复制的抑制作用研究
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Journal Article
新型中药制剂肃毒星对乙型肝炎病毒体外复制的抑制作用研究
2014
Overview
目的观察新型中药制剂肃毒星对野生和恩替卡韦（ETV）耐药乙型肝炎病毒（HBV）的抑制作用。方法以不同浓度（0、0．0005、0．001、0．005、0．01mg／m1）的肃毒星分别处理HepG2．2．15细胞（D基因型／ayw亚型野生HBV稳定复制细胞系）和HepG2．A64细胞（c基因型／adr亚型ETv耐药HBV稳定复制细胞系），作用时间4d。采用酶联免疫吸附试验检测细胞培养上清@HBsAg和HBeAg含量的变化；提取细胞内病毒核心颗粒，采用实时荧光定量PCR方法检测两种细胞内HBVDNA复制水平的变化。观察不同作用时间下，药物对HBVDNA的抑制作用。结果肃毒星对HepG2．2．1S细胞和HepG2．A64细胞内HBVDNA复制和HBsAg、HBeAg的分泌均有明显抑制作用，且具有药物剂量和时间依赖性，最高浓度（0．0lmg／m1）的肃毒星对HepG2．2．15细胞内病毒的抑制率达到80．83％，对HBsAg和HBeAg的抑制率分别达51．00％和64．05％；而对HepG2．A64细胞内HBVDNA抑制率为65．18％，对HBsAg和HBeAg的抑制率分别为32．8s％和73．86％。随肃毒星作用时间延长，其对HepG2．2．15细胞HBVDNA的抑制效果增强，但未显示对HepG2．A64细胞内ETV耐药型HBV株病毒抑制率增强的效果。结论体外细胞培养实验中，肃毒星不仅可有效抑制野生HBV复制和抗原分泌，而且对ETV耐药HBV的复制和抗原分泌也具有很好的抑制作用。
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