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cDNA clone and expression analysis of a-Tropomyosin during Japanese flounder （Parafichthys ofivaceus） metamorphosis
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cDNA clone and expression analysis of a-Tropomyosin during Japanese flounder （Parafichthys ofivaceus） metamorphosis
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cDNA clone and expression analysis of a-Tropomyosin during Japanese flounder （Parafichthys ofivaceus） metamorphosis
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Journal Article
cDNA clone and expression analysis of a-Tropomyosin during Japanese flounder （Parafichthys ofivaceus） metamorphosis
2014
Overview
Tropomyosin （TM） plays a critical role in skeletal and cardiac muscle development and function. To assess the fimctional significance of a-TM in Japanese flounder （Paralichthys olivaceus） development and metamorphosis, cDNA from Japanese flounder was cloned and a-TM mRNA measured during development and metamorphosis. The full-length cDNA is 1 191 bp, including a 5＇- untranslated region of 114 bp, a Y-UTR of 222 bp, and an open reading frame of 855 bp encoding a polypeptide of 284 amino acids. Real-time quantitative PCR revealed that a-TM mRNA is initially expressed in unfertilized ovum, indicating the a-TM gene is maternal. Relatively low mRNA levels were observed in different embryonic stages. A higher level of a-TM mRNA was detected 3 days post hatching （dph）, while the highest level was measured at 29 dph （metamorphic climax） after which it declined towards the end of metamorphosis. The expression of a-TM mRNA was up-regulated in thyroid hormone-treated larvae at 36 dph, but there was no marked difference
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