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子宫结合带干细胞的培养与鉴定
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子宫结合带干细胞的培养与鉴定
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Journal Article
子宫结合带干细胞的培养与鉴定
2015
Overview
目的建立子宫结合带干细胞（uJZSCs）的分离培养及鉴定方法。方法将手术中无菌取得的子宫内肌层即子宫结合带用胰酶和胶原酶Ⅰ进行消化、培养、扩增。采用倒置显微镜观察细胞形态,流式细胞仪进行表型分析及存活率判断,CCK-8法判断其生长特性,并在体外诱导其成脂、成骨、成软骨分化。结果所获细胞传代后能迅速贴壁,呈长梭形;流式细胞仪检测结果显示,表面标志物CD90、CD73、CD105、CD29、CD44、CD13、CD166、HLAABC呈阳性表达,而CD34、CD45、CD14、HLA-DR、CD19呈阴性表达。CCK-8生长曲线呈S形。诱导分化实验结果显示,uJZSCs具有成脂、成骨和成软骨的分化能力。结论 uJZSCs具有很强的增殖扩增能力,可作为间充质干细胞的来源。
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