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血清可溶性CD30在电离辐射剂量估算中的价值
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Journal Article
血清可溶性CD30在电离辐射剂量估算中的价值
2016
Overview
目的探讨血清可溶性CD 3 0（s CD 3 0）作为电离辐射剂量估算的生物指标的可行性。方法健康雄性C57BL/6小鼠300只,随机分为对照组及1、3、5、7Gy辐照组（n=60）。辐照组小鼠接受60Coγ射线一次性全身辐照,剂量率为0.78Gy/min。各组分别于辐照后24、48、72h留取血清样本（每组每时间点20只小鼠）,采用定量抗体芯片检测s CD30浓度,并通过回归分析法分析血清s CD30浓度与辐照剂量的相关性。结果未辐照小鼠s CD30正常值范围为121.2±25.6pg/ml;辐照后24、48、72h,血清s CD30浓度随辐射剂量增大而降低。回归分析显示,血清s CD30浓度与辐照剂量具有明显的剂量-效应关系（R~2=0.9913）,其回归方程为y=1.1489x~2-21.139x＋121.44。结论小鼠血清s CD30表达基线稳定,其浓度变化与急性辐照剂量相关,可作为γ电离辐射剂量估算的生物指标。
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