Journal Article

Species and Biotype Analysis of Brucella Strains solated from Cattle and Sheep in Xinjiang

Yi Xinping Ma Xiaojing Ye Feng Li Hongbo Liu Liya Tuerhong Nuer Zhang Yang Ma Junjie Wang Lijian Zhong Qi

2016

Overview

[ Objective] The paper aimed to understand the epidemiological characteristics of Brucella strains in Xinjiang and then provide an available integrated measure to prevent and control brucellosis. [ Method ] Eleven suspected Brucella strains were isolated by traditional methods, which were further identified by AMOS-PCR assay. Conventional biochemical tests were carried out to identify the biological subtype of sheep Brucella. [ Result] Nine strains were all B. meliten- s/s, and biological test indicated that all of them were B. melitensis biotype 3. [ Conclusion] B. melitensis biotype 3 was the predominant strain of Brucella in Xin- jiang, and AMOS-PCR assay could be applied safely and quickly as an assistant tool to detect Brucella. The results of molecular epidemiology laid a foundation for updating prevention and control strategy against brucellosis in Xinjiang.

• Subject: 分子流行病学 / 布氏杆菌病 / 布鲁氏菌病 / 新疆型 / 流行病学特征 / 生化检测 / 羊 / 菌株分离