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高同型半胱氨酸血症对急性ST段抬高型心肌梗死患者淋巴细胞KV1.3-CaN-NFAT信号通路的影响
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高同型半胱氨酸血症对急性ST段抬高型心肌梗死患者淋巴细胞KV1.3-CaN-NFAT信号通路的影响
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高同型半胱氨酸血症对急性ST段抬高型心肌梗死患者淋巴细胞KV1.3-CaN-NFAT信号通路的影响
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Journal Article
高同型半胱氨酸血症对急性ST段抬高型心肌梗死患者淋巴细胞KV1.3-CaN-NFAT信号通路的影响
2016
Overview
目的观察不同水平高同型半胱氨酸血症（Hhcy）对急性ST段抬高型心肌梗死（STEMI）患者淋巴细胞内电压门控性钾离子通道（KV1.3通道）-钙调神经磷酸酶（CaN）-活化T细胞核因子（NFAT）-炎性因子（IL-6、TNF-α）mRNA及蛋白表达水平的影响。方法入选STEMI患者90例,根据血浆Hcy水平分为3组：实验A组（STEMI组,Hcy〈15μmol/L,n=30）、实验B组（STEMI＋轻度Hhcy组,Hcy 15~30μmol/L,n=30）、实验C组（STEMI＋中度Hhcy组,Hcy〉30μmol/L,n=30）,另选取健康体检者30例为对照组。抽取血标本后使用全自动生化仪测定血浆Hcy浓度,密度梯度离心法提取淋巴细胞,RT-PCR检测KV1.3、CnAα、NFAT1、IL-6、TNF-αmRNA的表达,Western blot检测KV1.3、CnAα、NFAT1蛋白的表达。结果各实验组较对照组KV1.3、CnAα和NFAT1的mRNA和蛋白表达水平升高（P〈0.05或P〈0.01）,各实验组间两两比较显示,实验B组较实验A组表达水平升高（P〈0.05或P〈0.01）,实验C组较实验B组和实验A组表达水平升高（P〈0.05或P〈0.01）。各实验组IL-6和TNF-α的mRNA表达水平高于对照组（P〈0.01）,各实验组间两两比较显示,实验B组较实验A组表达水平升高（P〈0.01）,实验C组较实验B组和实验A组表达水平升高（P〈0.01）。实验组血浆Hcy水平与KV1.3mRNA和蛋白表达之间呈正相关,相关系数分别为r=0.503（P=0.000）、r=0.726（P=0.000）。结论血浆Hcy浓度水平越高,STEMI患者淋巴细胞内KV1.3-CnAα-NFAT1的mRNA和蛋白表达水平及炎性因子IL-6、TNF-α的mRNA表达水平越高,这一信号通路可能是Hcy加重STEMI炎症反应的机制之一。
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