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晚期氧化蛋白产物诱导HaCaT细胞凋亡的实验研究
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晚期氧化蛋白产物诱导HaCaT细胞凋亡的实验研究
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Journal Article
晚期氧化蛋白产物诱导HaCaT细胞凋亡的实验研究
2016
Overview
目的探讨晚期氧化蛋白产物（AOPPs）对HaCaT细胞凋亡的影响及其可能的作用机制。方法人血清白蛋白（HSA）与次氯酸钠溶液反应制成AOPPs。MTT法检测经不同浓度（0、50、100、200、400μg/ml）AOPPs处理24h后HaCaT细胞的活力,以选择AOPPs的工作浓度。根据时间效应或浓度效应对HaCaT细胞进行分组处理,流式细胞技术检测细胞凋亡率,二氯荧光素双醋酸盐（DCFH-DA）法检测细胞内活性氧（ROS）水平,Western blotting技术检测细胞内NOX4、Bax及Bcl-2蛋白的表达。结果 400μg/ml AOPPs作用后HaCaT细胞活力较其他浓度明显降低（P〈0.05）。随AOPPs作用时间的延长和浓度的增加,HaCaT细胞凋亡率明显增加（P〈0.05）,细胞内ROS产生增多（P〈0.05）,NOX4和Bax蛋白表达量增加（P〈0.05）,Bcl-2蛋白表达量降低（P〈0.05）。结论 AOPPs可能通过NADPH氧化酶途径诱导HaCaT细胞凋亡。
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