Journal Article

Identification of Gardenoside and Sarsasapogenin from Qingwen Baidu Granules by TLC

Guo Shijin Wang Yanping Zhang Zhimei Fu shijun Xu Qianqian Zhang Ying Shen Zhiqiang

2016

Overview

[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.

• Subject: 快速检测 / 栀子甙 / 百度 / 皂苷元 / 硫酸溶液 / 菝葜 / 薄层色谱鉴别 / 颗粒