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艾烟对肺泡Ⅱ型上皮细胞A549线粒体膜电位及Bax/Bcl-2影响的研究
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艾烟对肺泡Ⅱ型上皮细胞A549线粒体膜电位及Bax/Bcl-2影响的研究
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Journal Article
艾烟对肺泡Ⅱ型上皮细胞A549线粒体膜电位及Bax/Bcl-2影响的研究
2016
Overview
目的：观察艾灸生成物对肺泡Ⅱ型上皮细胞A549线粒体膜电位（mitochondrial transmembrane potential,MTP）、Bax/Bcl-2 m RNA表达的影响,进一步研究艾灸生成物对A549细胞的氧化损伤作用。方法：采用气体采样滤盒采集艾灸生成的烟气颗粒物,用细胞培养液稀释后依次配制质量浓度为0.05 mg/mL、0.1 mg/mL、0.2 mg/mL、0.3 mg/mL、0.4 mg/mL的艾烟提取物（moxa smoke extract,MSE）溶液干预A549细胞。采用JC-1染色法检测细胞MTP,荧光定量聚合酶链反应（polymerase chain reaction,PCR）法检测细胞Bax/Bcl-2 mRNA的表达。结果：与正常对照组细胞相比,0.3 mg/mL和0.4 mg/mL MSE干预组细胞MTP显著降低（P〈0.01）,0.05 mg/mL、0.1 mg/mL和0.2 mg/mL MSE干预组细胞MTP无显著变化（P〉0.05）;与0.05 mg/mL MSE干预组细胞相比,0.1 mg/mL、0.2 mg/mL、0.3 mg/mL和0.4 mg/mL MSE干预组细胞MTP显著降低（均P〈0.05）;与0.1 mg/mL MSE干预组细胞比较,0.4 mg/mL MSE干预组细胞MTP显著降低（P〈0.01）。各浓度MSE干预组细胞Bax mRNA的表达与正常对照组相比均无显著性差异;细胞内Bcl-2 mRNA表达随MSE干预浓度的增加而减少,其中0.4 mg/mL和0.3 mg/mL MSE干预组细胞与正常对照组细胞Bcl-2 mRNA的表达相比显著降低（P〈0.05）,0.4 mg/mL MSE干预组细胞Bcl-2mRNA的表达与0.05 mg/mL MSE干预组细胞内Bcl-2 mRNA的表达相比显著降低（P〈0.05）。结论：艾烟升高到一定浓度时可以降低肺泡Ⅱ型上皮细胞A549细胞内MTP,减少凋亡抑制基因Bcl-2 mRNA的表达,氧化损伤可能是高浓度艾烟溶液引起细胞凋亡的重要机制,具体机制仍需进一步研究。
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