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不同艾灸持续时间对类风湿关节炎大鼠影响的实验研究
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不同艾灸持续时间对类风湿关节炎大鼠影响的实验研究
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Journal Article
不同艾灸持续时间对类风湿关节炎大鼠影响的实验研究
2017
Overview
目的：观察不同艾灸持续时间对类风湿关节炎（RA）大鼠影响的差异,评价灸量与灸效的关系。方法：将40只雄性Sprague-Dawley（SD）大鼠随机选取8只作为正常组,其他32只建立Ⅱ型胶原诱导的RA模型。造模成功后,将32只大鼠随机分为模型组、艾灸20 min组、艾灸40 min组和艾灸60 min组,每组8只。正常组不造模,不进行艾灸干预;模型组大鼠造模后不予艾灸干预;艾灸20 min组、艾灸40 min组和艾灸60 min组大鼠分别接受艾灸肾俞和足三里20 min、40 min和60 min治疗。6 d为1个疗程,疗程间休息1 d,共治疗3个疗程。治疗结束后,观察大鼠血清中白介素（IL）-1β和肿瘤坏死因子（TNF）-α水平、关节炎指数（AI）评分、足趾容积和滑膜炎症病理学评分。结果：治疗前,与正常组比较,模型组大鼠血清IL-1β和TNF-α水平升高,足趾容积增加,组间差异均有统计学意义（均P〈0.01）。与模型组比较,艾灸20 min组、艾灸40 min组和艾灸60 min组血清IL-1β、TNF-α水平、足趾容积和AI评分均显著下降（P〈0.05 or P〈0.01）。与艾灸20 min组和艾灸60 min组比较,艾灸40 min组血清IL-1β和TNF-α水平、足趾容积和AI评分下降更明显,组间有统计学差异（P〈0.05 or P〈0.01）。艾灸20 min组和艾灸60 min组比较,血清IL-1β、TNF-α水平、AI评分和足趾容积差异均无统计学意义（均P〉0.05）。艾灸20 min组、艾灸40 min组和艾灸60 min组的滑膜组织病理的改变比较,艾灸40 min组滑膜组织病理改善最明显。结论：艾灸40 min治疗RA大鼠的作用效果比艾灸20 min及艾灸60 min更明显,说明艾灸治疗持续时间是影响艾灸治疗效果的主要因素。
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