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EVALUACIÓN IN VITRO DEL POTENCIAL PROBIÓTICO DE BACTERIAS ÁCIDO LÁCTICAS AISLADAS DE SUERO COSTEÑO IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF LACTIC BACTERIA ACID ISOLATED FROM COASTAL SERUM
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EVALUACIÓN IN VITRO DEL POTENCIAL PROBIÓTICO DE BACTERIAS ÁCIDO LÁCTICAS AISLADAS DE SUERO COSTEÑO IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF LACTIC BACTERIA ACID ISOLATED FROM COASTAL SERUM
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EVALUACIÓN IN VITRO DEL POTENCIAL PROBIÓTICO DE BACTERIAS ÁCIDO LÁCTICAS AISLADAS DE SUERO COSTEÑO IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF LACTIC BACTERIA ACID ISOLATED FROM COASTAL SERUM
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Journal Article
EVALUACIÓN IN VITRO DEL POTENCIAL PROBIÓTICO DE BACTERIAS ÁCIDO LÁCTICAS AISLADAS DE SUERO COSTEÑO IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF LACTIC BACTERIA ACID ISOLATED FROM COASTAL SERUM
2010
Overview
Un grupo de 53 bacterias ácido lácticas (BAL) aisladas del suero costeño, fueron sometidas a estudios preliminares in vitro simulando las condiciones del tracto digestivo, para determinar sus características como potenciales probióticos: se evaluó la tolerancia a pH ácido (MRS pH: 2,0) y sales biliares (MRS con sales biliares al 0,3%) y posteriormente se determinó la población sobreviviente como log UFC/ml. A las cepas tolerantes a las condiciones mencionadas, se les determinó la resistencia a 14 antibióticos de uso comercial, se evaluó la adhesión a mucus intestinal y la producción de ácido láctico por cromatografía líquida de alta precisión (HPLC). Se encontró que 54,7% de las BAL evaluadas son tolerantes a las condiciones de pH ácido y 49,1 a 0,3% de sales biliares con una población de 10(6) log UFC/ml en promedio. Siete cepas fueron seleccionadas por presentar sensibilidad a vancomicina antibiótico de importancia epidemiológica y se adhirieron a mucus intestinal, reuniendo las condiciones requeridas para considerarse como potencialmente probióticas. Adicionalmente la cuantificación de ácido láctico para las cepas seleccionadas presentó un rango entre 0,13 ± 0,05 y 1,0 ± 0,08 g/l.A group of 53 lactic acid bacteria (LAB) isolated from costal serum were evaluated with preliminary in vitro studies simulating the conditions of the digestive tract, to determine their characteristics as potential probiotics; acid pH tolerance (MRS pH: 2.0) and bile salts (MRS with 0.3% bile salts) and subsequently the surviving population was determined as log CFU/ml. Strains tolerant to such conditions were evaluated for resistance to 14 antibiotics of commercial use; we assessed adherence to intestinal mucus and the production of lactic acid by high performance liquid chromatography (HPLC). It was shown that 54.7% of the LAB evaluated were tolerant to acid pH conditions and 49.1 to 0.3% bile salts with a population of 10(6) log CFU/ml on average. Seven strains were selected by antibiotic vancomycin tenderness of epidemiological importance and adhered to intestinal mucus, meeting the requirements to be considered as potentially probiotic. Additionally, the quantification of lactic acid in the selected strains showed a range between 0.13 and 1.0 ± 0.05 ± 0.08 g/l.
Publisher
Universidad de Antioquia
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