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Effect of heparin, estrogen on Epididymal sperm capacitation and in Vitro Fertilization in Iraqi sheep
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Effect of heparin, estrogen on Epididymal sperm capacitation and in Vitro Fertilization in Iraqi sheep
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Journal Article
Effect of heparin, estrogen on Epididymal sperm capacitation and in Vitro Fertilization in Iraqi sheep
2020
Overview
This study was designed to study the capacitation of caudal spermatozoa in vitro by different
levels of heparin, estrogen for in vitro fertilization (IVF) in the Iraqi local sheep. Results of
sperm capcitation (massive motility percentage) by applying three levels of heparin (50, 100,
150) IU in relation to breeding season showed no significant differences during breeding
season with the three levels, which were (82.23 ± 0.58), While results by applying the higher
level of heparin (150) IU out of breeding season showed significantly (P<0.05) more and active
motility Which were (62.07 ± 0.62) than the other levels (56.85 ± 0.61). At the same time
applications result of three estrogen levels (20, 40, 60) mg on sperm capcitation showed
Positive relationship between concentrations and (massive motility percentage), which showed
the highest concentration gave the best results during and out of breeding season (87.20 ±
0.60) and (65.86 ± 0.62) respectively. with significant differences (P<0.05) between the three
levels. While the results of the in vitro fertilization (IVF) index which reflected the sperm
capcitation were recorded with the highest estrogen concentration during breeding season
(22.70 %) compared with the best heparin level (20 %) while she was (10.45 %), (8.70 %) out
of breeding season Sequentially, in which the highest estrogen concentration gives a best
capacitation and IVF index level compared with the high heparin level over the year.
Publisher
University of Baghdad, College of Agriculture
Subject
