Journal Article

New gSSR and EST-SSR markers reveal high genetic diversity in the invasive plant Ambrosia artemisiifolia L. and can be transferred to other invasive Ambrosia species

Agroécologie [Dijon] ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Bailly, Geraldine, Pernin, Fanny, BASF France SAS; EU COST Action FM203; Carl Trygger Foundation for Scientific Research (CTS); Short Term Scientific Mission [COST-STSM-FA1203-21009, CTS 14.425][e-mail], Le Corre, Valérie, Délye, Christophe, Chauvel, Bruno, Scalone, Romain, Causse, Romain, Sustainable Agriculture Department ; BASF Agro SAS, Meyer, Lucie, Department of Crop Production Ecology ; Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU)

2017

Overview

Ambrosia artemisiifolia L., (common ragweed), is an annual invasive and highly troublesome plant species originating from North America that has become widespread across Europe. New sets of genomic and expressed sequence tag (EST) based simple sequence repeats (SSRs) markers were developed in this species using three approaches. After validation, 13 genomic SSRs and 13 EST-SSRs were retained and used to characterize the genetic diversity and population genetic structure of Ambrosia artemisiifolia populations from the native (North America) and invasive (Europe) ranges of the species. Analysing the mating system based on maternal families did not reveal any departure from complete allogamy and excess homozygosity was mostly due the presence of null alleles. High genetic diversity and patterns of genetic structure in Europe suggest two main introduction events followed by secondary colonization events. Cross-species transferability of the newly developed markers to other invasive species of the Ambrosia genus was assessed. Sixty-five percent and 75% of markers, respectively, were transferable from A. artemisiifolia to Ambrosia psilostachya and Ambrosia tenuifolia. 40% were transferable to Ambrosia trifida, this latter species being seemingly more phylogenetically distantly related to A. artemisiifolia than the former two.

• Publisher: Public Library of Science,CCSD
• ISBN: 0004013141000