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Carbon nanodots combined with loop-mediated isothermal amplification
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Journal Article
Carbon nanodots combined with loop-mediated isothermal amplification
2022
Overview
The spread of African swine fever virus (ASFV) caused huge economic costs, so early detection is particularly important. Here, we established a fluorescence biosensor based on carbon nanodots (CNDs) and loop-mediated isothermal amplification (LAMP) to ultra-sensitively detect ASFV. LAMP with high efficiency produced a large amount of pyro phosphoric acid and caused pH change in a short time. CNDs with strong light stability had a large fluorescence response at the emission wavelength of 585.5 nm to small pH change by the excitation wavelength of 550 nm. The biosensor realized \"turn-off-on\" mode for ASFV detection with the detection limit as low as 15.21 copies [mu]L.sup.-1. In addition, the biosensor had high accuracy in the actual sample assay. Therefore, the biosensor achieved rapid, sensitive, low-cost, and simple detection for ASFV. Moreover, the biosensor broadened the detection pathway of LAMP as a tool with great development prospect. Graphical abstract
Publisher
Springer
Subject
