Asset Details
Do you wish to reserve the book?
Determination of Fumonisin Bsub.1 by Aptamer-Based Fluorescence Resonance Energy Transfer
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Determination of Fumonisin Bsub.1 by Aptamer-Based Fluorescence Resonance Energy Transfer
Do you wish to request the book?
Determination of Fumonisin Bsub.1 by Aptamer-Based Fluorescence Resonance Energy Transfer
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Determination of Fumonisin Bsub.1 by Aptamer-Based Fluorescence Resonance Energy Transfer
2022
Overview
Fumonisin FB is produced by Fusarium moniliforme Sheld, of which FB[sub.1] is the most common and the most toxic. The establishment of a rapid detection method is an important means to prevent and control FB[sub.1] pollution. A highly sensitive fluorescent sensor based on an aptamer for the rapid detection of fumonisin B[sub.1] (FB[sub.1]) in corn was established. In this study, 5-carboxyfluorescein (FAM) was labeled on the aptamer of FB[sub.1] (F10). F10 was adsorbed on the surface of graphene oxide (GO) by π-π stacking. The FAM fluorescence signal could be quenched by fluorescence resonance energy transfer between fluorescent molecules and graphene oxide (GO). In the presence of FB[sub.1], the binding efficiency of the aptamer to GO was reduced. Therefore, the content of FB[sub.1] in corn samples was determined by fluorescence measurements of mixed FAM-labeled F10, GO and corn samples. This method had a good linear relationship in an FB[sub.1] concentration range of 0–3000 ng/mL. The equation was y = 0.2576x + 10.98, R[sup.2] = 0.9936. The limit of detection was 14.42 ng/mL, and the limit of quantification was 43.70 ng/mL. The recovery of a spiked standard in the corn sample was 89.13–102.08%, and the time of detection was 30 min.
Publisher
MDPI AG
Subject
