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以多套式聚合酶鏈反應同時檢測臺中市附近環境水中熱不穩定性腸毒素LTI和熱穩定性腸毒素STⅡ之大腸桿菌
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以多套式聚合酶鏈反應同時檢測臺中市附近環境水中熱不穩定性腸毒素LTI和熱穩定性腸毒素STⅡ之大腸桿菌
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以多套式聚合酶鏈反應同時檢測臺中市附近環境水中熱不穩定性腸毒素LTI和熱穩定性腸毒素STⅡ之大腸桿菌
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Journal Article
以多套式聚合酶鏈反應同時檢測臺中市附近環境水中熱不穩定性腸毒素LTI和熱穩定性腸毒素STⅡ之大腸桿菌
1998
Overview
The enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) cells include heat labile (LT) and heat stable toxins (ST). These toxins cause diarrheal in humans and domestic animals. In this study, we combined LT I and ST II gene specific PCR primers into a multiplex PCR systemand used this system for the simultaneous detection of LT I and ST II ETEC cells in tap water and environmental waters. Using Chromocult coliform agar (CCA), we found that tap water and underground water were contaminated with 0-10 0 CFU/ml of E. coli and coliform cells while the waters from four rivers near Taichung City were contaminated with 10 2 - 10 4 CFU/ml of E. coli and coliform cells. Furthermore, three of the four river waters were contaminated with LT I/ST II ETEC cells. The high levels of microfloral contamination in these river waters near an urban area should be attended to urgently.
Publisher
衛生福利部食品藥物管理署
Subject
