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Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex
Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex
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Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex
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Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex
Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex

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Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex
Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex
Journal Article

Transcriptional, Posttranscriptional, and Posttranslational Regulation ofSHOOT MERISTEMLESSGene Expression in Arabidopsis Determines Gene Function in the Shoot Apex

2015
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Overview
The activity ofSHOOT MERISTEMLESS (STM)is required for the functioning of the shoot apical meristem (SAM).STMis expressed n the SAM but is down-regulated at the site of leaf initiation.STMis also required for the formation of compound leaves. However, how the activity ofSTMis regulated at the transcriptional, posttranscriptional, and posttranslational levels is poorly understood.We previously found two conserved noncoding sequences in the promoters ofSTM-like genes across angiosperms, the K-box and the RB-box. Here, we characterize the function of the RB-box in Arabidopsis (Arabidopsis thaliana). The RB-box, along with the K-box, regulates the expression ofSTMin leaf sinuses, which are areas on the leaf blade with meristematic potential. The RB-box also contributes to restrictSTMexpression to the SAM. We identified FAR1-RELATED SEQUENCES-RELATED FACTOR1 (FRF1) as a binding factor to the RB-box region. FRF1 is an uncharacterized member of a subfamily of four truncated proteins related to the FAR1-RELATED SEQUENCES factors. Internal deletion analysis of theSTMpromoter identified a region required to repress the expression ofSTMin hypocotyls. Expression ofSTMin leaf primordia under the control of theJAGGEDpromoter produced plants with partially undifferentiated leaves.We further found that the ELK domain has a role in the posttranslational regulation of STM by affecting the nuclear localization of STM.