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Expression Patterns ofArabidopsis DRGGenes: Promoter‐GUS Fusions, Quantitative Real‐Time PCR, and Patterns of Protein Accumulation in Response to Environmental Stresses
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Expression Patterns ofArabidopsis DRGGenes: Promoter‐GUS Fusions, Quantitative Real‐Time PCR, and Patterns of Protein Accumulation in Response to Environmental Stresses
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Expression Patterns ofArabidopsis DRGGenes: Promoter‐GUS Fusions, Quantitative Real‐Time PCR, and Patterns of Protein Accumulation in Response to Environmental Stresses
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Journal Article
Expression Patterns ofArabidopsis DRGGenes: Promoter‐GUS Fusions, Quantitative Real‐Time PCR, and Patterns of Protein Accumulation in Response to Environmental Stresses
2008
Overview
DRGs are very highly conserved GTP‐binding proteins. All eukaryotes containDRG1andDRG2orthologs.Arabidopsishas threeDRGs:AtDRG1(At4g39520),AtDRG2(At1g17470), andAtDRG3(At1g72660).DRG2andDRG3encode proteins that are 95% identical; identity betweenDRG1andDRG2/3is 55%. The focus of this article is expression ofArabidopsis DRGs.DRG1andDRG2promoter‐GUS constructs showed similar spatial expression in seedlings and mature organs, but gene‐specific differences were noted. Quantitative real‐time PCR experiments indicated similar levels ofDRG1andDRG2mRNA accumulation in most tissues.DRG3transcripts were very low in all tissues. Heat stress at 37°C led to a 10‐fold increase inDRG1transcripts and a 1000‐fold increase inDRG3transcripts.DRG1antibodies recognized a 43‐kD protein, andDRG2antibodies recognized bands at 30, 43, and 45 kD. Plants were exposed to stresses (salt, heat, cold, UV light, osmotic, and other stresses) and examined by Western blotting. Only heat stress caused detectable changes. Heat did not affectDRG1, butDRG2and a 72‐kD protein recognized byDRG2antibodies both increased. The modest changes inDRGmRNA and protein levels seen here suggest that other types of regulation, such as altered subcellular localization, may be important for their cellular functions.
Publisher
The University of Chicago Press
Subject
