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Electron Transfer Dissociation of All Ions at All Times, MSsup ETD, in a Quadrupole Time-of-Flight (Q-ToF) Mass Spectrometer
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Electron Transfer Dissociation of All Ions at All Times, MSsup ETD, in a Quadrupole Time-of-Flight (Q-ToF) Mass Spectrometer
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Electron Transfer Dissociation of All Ions at All Times, MSsup ETD, in a Quadrupole Time-of-Flight (Q-ToF) Mass Spectrometer
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Journal Article
Electron Transfer Dissociation of All Ions at All Times, MSsup ETD, in a Quadrupole Time-of-Flight (Q-ToF) Mass Spectrometer
2017
Overview
Data-independent mass spectral acquisition is particularly powerful when combined with ultra-performance liquid chromatography (LC) that provides excellent separation of most components present in a given sample. Data-independent analysis (DIA) consists of alternating full MS scans and scans with fragmentation of all ions within a selected m/z range, providing precursor masses and structure information, respectively. Fragmentation spectra are acquired either by sequential isolation and fragmentation of sliding m/z ranges or fragmenting all ions entering the MS instrument with no ion isolation, termed broadband DIA. Previously, broadband DIA has only been possible using collision induced dissociation (CID). Here, we report the use of electron transfer dissociation (ETD) as the fragmentation technique in broadband DIA instead of traditional collision induced dissociation (CID) during MSsup E. In this approach, which we refer to as MSsup ETD, we implement the inherent benefits provided by ETD, such as discrimination of leucine and isoleucine, in a DIA setup. The combination of DIA analysis and ETD fragmentation with supplemental CID energy provides a powerful platform to obtain information on all precursors and their sequence from a single experiment. .
