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Gated rotation mechanism of site-specific recombination by phiC31 integrase
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Gated rotation mechanism of site-specific recombination by phiC31 integrase
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Journal Article
Gated rotation mechanism of site-specific recombination by phiC31 integrase
2012
Overview
Integrases, such as that of the Streptomyces temperate bacteriophage ...C31, promote site-specific recombination between DNA sequences in the bacteriophage and bacterial genomes to integrate or excise the phage DNA. ...C31 integrase belongs to the serine recombinase family, a large group of structurally related enzymes with diverse biological functions. It has been proposed that serine integrases use a \"subunit rotation\" mechanism to exchange DNA strands after double-strand DNA cleavage at the two recombining att sites, and that many rounds of subunit rotation can occur before the strands are religated. We have analyzed the mechanism of ...C31 integrase-mediated recombination in a topologically constrained experimental system using hybrid \"phes\" recombination sites, each of which comprises a ...C31 att site positioned adjacent to a regulatory sequence recognized by Tn3 resolvase. The topologies of reaction products from circular substrates containing two phes sites support a right-handed subunit rotation mechanism for catalysis of both integrative and excisive recombination. Strand exchange usually terminates after a single round of 180... rotation. However, multiple processive \"360... rotation\" rounds of strand exchange can be observed, if the recombining sites have nonidentical base pairs at their centers. We propose that a regulatory \"gating\" mechanism normally blocks multiple rounds of strand exchange and triggers product release after a single round. (ProQuest: ... denotes formulae/symbols omitted.)
Publisher
National Academy of Sciences
Subject
