Asset Details
Do you wish to reserve the book?
Genotyping of Macrophage Migration Inhibitory Factor (MIF) CATT^sub 5âeuro\8^ Repeat Polymorphism by Denaturing High-Performance Liquid Chromatography (DHPLC)
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Genotyping of Macrophage Migration Inhibitory Factor (MIF) CATT^sub 5âeuro\8^ Repeat Polymorphism by Denaturing High-Performance Liquid Chromatography (DHPLC)
Do you wish to request the book?
Genotyping of Macrophage Migration Inhibitory Factor (MIF) CATT^sub 5âeuro\8^ Repeat Polymorphism by Denaturing High-Performance Liquid Chromatography (DHPLC)
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Genotyping of Macrophage Migration Inhibitory Factor (MIF) CATT^sub 5âeuro\8^ Repeat Polymorphism by Denaturing High-Performance Liquid Chromatography (DHPLC)
2013
Overview
Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine expressed in many different cell types and implicated in the pathogenesis of numerous acute and chronic inflammatory diseases. Variable Number of Tandem Repeat (VNTR) CATT^sub 5â[euro]\"8^ at position â '794 in the promoter of the MIF gene has been associated with several human pathological conditions. Different methods for genotyping the CATT tetranucleotide repeats have been described. Here, we report, for the first time, the complete characterization of the CATT^sub 5â[euro]\"8^ repeat polymorphism using exclusively the denaturing high-performance liquid chromatography (DHPLC) technique under partially denaturing conditions. This approach, based on a step-by-step DHPLC protocol, allowed the accurate determination of all the homozygous and heterozygous genotypes in 350 DNA samples from control subjects. The results were validated by comparison to DNA sequencing, and the DHPLC approach was accurate, sensitive, and highly reproducible. Data from the current study demonstrate that this method of analysis by DHPLC may represent a powerful and sensitive alternative tool for a rapid and efficient genotyping of short tandem repeats presenting a limited number of alleles.[PUBLICATION ABSTRACT]
Publisher
Springer Nature B.V
Subject
