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Casting a line to trailing cells: a simple mechanism for polarizing signalling in the posterior lateral line primordium
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Casting a line to trailing cells: a simple mechanism for polarizing signalling in the posterior lateral line primordium
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Casting a line to trailing cells: a simple mechanism for polarizing signalling in the posterior lateral line primordium
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Journal Article
Casting a line to trailing cells: a simple mechanism for polarizing signalling in the posterior lateral line primordium
2018
Overview
Background: The zebrafish posterior lateral line primordium (PLLp) is a group of ~150 cells which spearheads the development of the lateral line by migrating along the length of the embryo, periodically depositing epithelial rosettes which serve as sense organ precursors. The PLLp is patterned by juxtaposed and mutually inhibitory Wnt and FGF signalling systems. Wnt in leading cells drives the expression of both FGF ligands and FGF signalling inhibitors. FGF ligand therefore activates receptors in more trailing cells, promoting rosette formation. However, the mechanisms by which this polarity is established and then maintained are incompletely understood. Methods: We used high resolution imaging in live zebrafish embryos mosaically labelled with a membrane GFP to characterize the formation and release of extracellular vesicles during the development of the PLLp. Results: Using high resolution timelapse imaging, we show that leading cells extend long vesicle-bearing fillopodial protrusions, similar to cytonemes, towards trailing cells. Small extracellular vesicles released by these protrusions are taken up by trailing cells and rapidly transported apically, where FGF is known to accumulate in a microlumenal compartment of the epithelial rosette. The extension of these protrusions is sensitive to inhibition of HSPG sulfation, a manipulation also known to prevent an effective FGF response in trailing cells. Furthermore, we show that the direction of extension of these protrusions is highly correlated with the direction and speed of cell migration. Summary/Conclusion: We propose that extracellular-vesicle mediated signalling is, at least in part, responsible for delivering signals from leading cells to trailing cells to in a manner intrinsically tied to the directionality of PLLp movement.
Publisher
John Wiley & Sons, Inc
Subject
