Asset Details
Do you wish to reserve the book?
Plasma exosome count is correlated with grade of lung cancer stages; comparison between pulmonary vein versus peripheral vein
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Plasma exosome count is correlated with grade of lung cancer stages; comparison between pulmonary vein versus peripheral vein
Do you wish to request the book?
Plasma exosome count is correlated with grade of lung cancer stages; comparison between pulmonary vein versus peripheral vein
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Plasma exosome count is correlated with grade of lung cancer stages; comparison between pulmonary vein versus peripheral vein
2018
Overview
Background: Lung cancer-derived exosomes are spill out of primary cancer and circulate in the peripheral blood after passing through the pulmonary drainage vein. But, there are no studies that performed quantitative comparisons of exosome using peripheral venous (PP) blood and pulmonary venous (PV) in each pathological stage of lung cancer patients. We explored the relationship between stage of patients undergoing surgery and exosomes according to the blood sampling site. Methods: Five rabbits were used for both normal and lung cancer model. The cancer model was constructed using in a non-invasive manner using real-time CT fluoroscopy-guided VX2 injection in rabbit lung. Five normal and 35 lung cancer patients were included, who underwent lung cancer surgery. Preclinical blood was collected from a PP in ear vein and PV. For patient, 3 ml of blood was collected from the lobar PV of the primary lung cancer site, and PP-blood from median cubital vein during surgery. Quantitative analysis was performed by nanoparticle tracking assay, CD63 enzyme-linked immunosorbent assay, and western blotting. Results: In preclinical, lung cancer lesion was confirmed by PET/CT image 2 weeks after injection, and solitary nodule was well formed. Exosome-count was no significant difference between PP and PV exosomes in normal (p = 0.8), However, it was increased in PP of lung cancer compared to normal (p = 0.012), and more increased in PV of cancer model (p = 0.0012). In patients, exosome counts and CD63 in PP were increased than control (p < 0.0001), and more significantly increased in PV than PP of patients (p < 0.0001). We investigated whether the increase of exosomes is related to stage and tumour size, and statistically that PV is more closely related than PP (p < 0.0001). Summary/Conclusion: We first compared PV and PP-derived exosomes in lung cancer patients and found that they were correlated with the stage. These results suggest that PV exosomes may provide more accurate results than conventional pathologic tests in patients undergoing lung cancer surgery.
Publisher
John Wiley & Sons, Inc
Subject
