Asset Details
Do you wish to reserve the book?
Development and Application of a 3D-Printed Microfluidic Device for Multi-Modal Detection of Nitric Oxide and ATP
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Development and Application of a 3D-Printed Microfluidic Device for Multi-Modal Detection of Nitric Oxide and ATP
Do you wish to request the book?
Development and Application of a 3D-Printed Microfluidic Device for Multi-Modal Detection of Nitric Oxide and ATP
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Dissertation
Development and Application of a 3D-Printed Microfluidic Device for Multi-Modal Detection of Nitric Oxide and ATP
2022
Overview
This dissertation details the use of 3D-printing for the analysis of cellular messengers. First, the significance of culturing cells on a 3D matrix and under flowing conditions is described. Electrospinning is used to fabricate an extracellular matrix mimic for 3D culture. RAW 264.7 macrophages are cultured on fibrous inserts and placed in a 4-insert 3D-printed cell culture device to analyze M1 activation (via TNF-a) in response to flowing or static media containing lipopolysaccharide. Macrophages on polycaprolactone and silk fibroin fibers are studied, with macrophages cultured on ~500 nm silk fibers exhibiting the most in-vivo-like response. Then, the creation of the first 3D-printed microfluidic in-line amperometric detector is described. The design incorporates a three-electrode set-up made possible by threading fabricated and commercially available threaded electrodes into the 3D-printed flow cell. To demonstrate in-line capabilities, the device is modified to include a mixing T for introduction of reagents for chemiluminescent detection of ATP (via the luciferin/luciferase reaction), leading to a single 3D-printed device that can be used to detect norepinephrine and ATP by amperometry and chemiluminescence. Next, the 3D-printed multi-modal device was optimized to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions prepared from whole blood. NO is detected with a platinum-black/Nafion coated gold working electrode. ATP is detected via chemiluminescence, with the luciferin/luciferase solution continuously pumped into an integrated double mixing T. The device is used to determine the levels of NO and ATP in normoxic and hypoxic red blood cells. The effect of storing red blood cells in a commonly used storage solution is also investigated by monitoring the production of NO and ATP over a three-week storage time. Finally, some preliminary results are presented of a possible configuration coupling the 3D-printed cell culture device to the in-line amperometric detector. The system can be used to study red blood cell-macrophage interactions in multiple sclerosis.
Publisher
ProQuest Dissertations & Theses
Subject
ISBN
9798845418616
