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Discovery of Novel Enzymatic Activities and Functions in Lipid Catabolism
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Discovery of Novel Enzymatic Activities and Functions in Lipid Catabolism
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Dissertation
Discovery of Novel Enzymatic Activities and Functions in Lipid Catabolism
2022
Overview
Mitochondria are highly involved in the control and regulation of diverse lipid species that are critical for cellular and mitochondrial activity, lipid membrane biosynthesis, signaling, and energy metabolism. Mitochondrial lipid metabolism is conducted by a network of enzymes that are organized throughout mitochondria, of which make ~11% of the known human mitochondrial proteome. The necessity of these enzymes is signified by the range of diseases that can stem from aberrant metabolism of specific mitochondrial lipids. Furthermore, new lipid classes implicated in the regulation of metabolism and/or mitochondrial activity directly continue to be discovered. In this thesis, I describe our work to determine the endogenous substrates and functions of certain uncharacterized mitochondrial proteins and the development of new technologies to better understand how lipid metabolism is controlled and regulated by mitochondria.
