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The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
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The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
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The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
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Dissertation
The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
2008
Overview
In eukaryotes, the genome is packaged into a DNA-protein complex called chromatin. As such, chromatin is intimately involved in the first step of gene expression, transcription. Chromatin and transcription are often studied using Drosophila melanogaster as a model organism, in part because the large polytene chromosomes in the salivary glands of Drosophila larvae facilitate examination of chromatin via microscopy. Genetic analysis of the Drosophila leg-arista-wing complex (lawc) gene suggests a role for the Lawc protein in chromatin-related processes. Lawc is a small, cysteine-rich protein that is present at most of the interbands of polytene chromosomes. Interbands are regions of open chromatin that contain active genes. In agreement with this observation, Lawc co-localizes with phosphorylated RNA polymerase IIo (Pol IIo) and it is recruited to transcribed loci after elongation by Pol IIo has begun. lawc mutants show decreased expression of many genes, but the levels of the elongating form of Pol IIo increase. The Lawc protein interacts with the nuclear proteasome regulator Drosophila REGγ (dREGγ) and dREGγ also co-localizes with Pol IIo on polytene chromosomes. In addition, a mutation in lawc interacts genetically with a mutation in a component of the proteasome. I conclude that Lawc is required for proper transcription by RNA polymerase II in a process that involves the nuclear proteasome.
Publisher
ProQuest Dissertations & Theses
Subject
ISBN
0549585478, 9780549585473
