Asset Details
Do you wish to reserve the book?
Characterization of the Skin Microbiome of Free-Ranging Bottlenose Dolphins (Tursiops truncatus) in a Dynamic Environment Using a Novel Technique
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Characterization of the Skin Microbiome of Free-Ranging Bottlenose Dolphins (Tursiops truncatus) in a Dynamic Environment Using a Novel Technique
Do you wish to request the book?
Characterization of the Skin Microbiome of Free-Ranging Bottlenose Dolphins (Tursiops truncatus) in a Dynamic Environment Using a Novel Technique
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Dissertation
Characterization of the Skin Microbiome of Free-Ranging Bottlenose Dolphins (Tursiops truncatus) in a Dynamic Environment Using a Novel Technique
2024
Overview
The objectives of this study are to determine if: 1) skin swabs from dolphin biopsy samples yield sufficient microbial DNA for 16S rRNA gene sequencing analyses, 2) the dolphin skin microbiome relates to the ambient water microbial community, 3) dolphin skin microbial community composition is affected by environmental attributes, and 4) sample handling affects DNA concentration or microbial community composition. These objectives will be answered by extracting, amplifying, sequencing, and analyzing skin swabs from biopsy samples collected from dolphins in Mobile Bay, Perdido Bay, and the Gulf of Mexico. It is hypothesized that: 1) dolphin skin biopsy swab samples will yield sufficient DNA to characterize the microbiome, differing from those in ambient water, 2) there will be variation in microbial community composition relative to environmental factors, 3) samples collected with cotton swabs and stored in RNAlater® will have different community compositions than samples that were collected with nylon flocked swabs and flash frozen in liquid nitrogen. This study tests a novel technique to analyze the skin microbiome of free-ranging animals without capture that will also preserve biopsy skin samples for additional studies. This information is important to assure the best methodology for future studies relating dolphin microbiome to ecology and health.
Publisher
ProQuest Dissertations & Theses
Subject
ISBN
9798383213834
