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THE RNA METAVIROME OF CULEX PIPIENS MOSQUITOES COLLECTED IN BUCHAREST
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THE RNA METAVIROME OF CULEX PIPIENS MOSQUITOES COLLECTED IN BUCHAREST
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Journal Article
THE RNA METAVIROME OF CULEX PIPIENS MOSQUITOES COLLECTED IN BUCHAREST
2025
Overview
Objectives. Culex pipiens mosquitoes are the vectors of West Nile virus (WNV) in Romania. Usutu virus - an emergent zoonotic mosquito-borne pathogen causing mortality in birds - was also detected in Cx. pipiens from Bucharest. Metavirome analysis conducted on vector populations facilitates the understanding of the wide diversity and abundance of arboviruses and insect-specific viruses (ISVs). This high-throughput sequencing study aimed to characterise the RNA metavirome of Cx. pipiens collected in Bucharest. Materials and methods. Female mosquito pools positive for WNV and collected in 2023 (2) and 2024 (9) were selected for analysis. RNA extracted from mosquito pool homogenates was used for metagenomic library preparation with Nextera XT and sequencing was performed on a MiSeq or NovaSeq 6000 system (Illumina). The resulting data were analysed using open-access platforms. Results and discussion. The most abundant RNA viruses detected are not yet classified at genus/family level (Hubei virga-like virus 2, Hubei mosquito virus 4, Culex Bunya-like virus, Culex Bunyavirus 2, Culex mononega-like virus 1, Culex negev-like virus 1, Anjon virus, Serbia picornalike virus 1, Culex associated luteo-like virus). Members of negative-sense single-stranded RNA virus families (Peribunyaviridae, Rhabdoviridae, Phasmaviridae, and Orthomyxoviridae), positivesense single-stranded RNA virus families (Flaviviridae, Virgaviridae, Iflaviridae, Nodaviridae, Luteoviridae, Picornaviridae, Solemoviridae, and Dicistroviridae), and double-stranded RNA virus families (Chrysoviridae and Totiviridae) were detected also. Whole genome sequences of WNV were recovered from seven samples. Phylogenetic analysis indicated lineage 2a. Conclusions. The role of the identified ISVs is unknown. It is speculated that they can modulate the transmission of human viral pathogens by interfering with replication, or that they represent the evolutionary basis for viruses of medical significance. Funding source: This work was carried in the frame of the Core Program within the National Research Development and Innovation Plan 2022-2027, carried out with the support of MCID, project no. 23 44.
