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NADPH regulates human NAD kinase, a NADP^sup +^-biosynthetic enzyme
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NADPH regulates human NAD kinase, a NADP^sup +^-biosynthetic enzyme
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Journal Article
NADPH regulates human NAD kinase, a NADP^sup +^-biosynthetic enzyme
2011
Overview
NAD kinase (NADK, EC 2.7.1.23) is the sole NADP^sup +^-biosynthetic enzyme that catalyzes phosphorylation of NAD^sup +^ to yield NADP^sup +^ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target. Although methods for expression and purification of human NADK have been previously established (Lerner et al. Biochem Biophys Res Commun 288:69-74, 2001), the purification procedure could be significantly improved. In this study, we improved the method for expression and purification of human NADK in Escherichia coli and obtained a purified homogeneous enzyme only through heat treatment and single column chromatography. Using the purified human NADK, we revealed a sigmoidal kinetic behavior toward ATP and the inhibitory effects of NADPH and NADH, but not of NADP^sup +^, on the catalytic activity of the enzyme. These inhibitory effects provide insight into the regulation of intracellular NADPH synthesis. Furthermore, these attributes may provide a clue to design a novel drug against Mycobacterium tuberculosis in which this bacterial NADK is potently inhibited by NADP^sup +^.[PUBLICATION ABSTRACT]
Publisher
Springer Nature B.V
Subject
