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Mussel-inspired surface modification of magnetic graphite nanosheets composite for efficient Candida rugosa lipase immobilization
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Mussel-inspired surface modification of magnetic graphite nanosheets composite for efficient Candida rugosa lipase immobilization
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Journal Article
Mussel-inspired surface modification of magnetic graphite nanosheets composite for efficient Candida rugosa lipase immobilization
2015
Overview
By the facile adhesion way, the novel composite complex by polydopamine (PDA) and magnetic graphite nanosheets (Fe sub(3)O sub(4)GNSs) has been successfully synthesized. The resulting composite was characterized by means of scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, and Raman spectra, X-ray diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. Meanwhile, the PDA functionalized Fe sub(3)O sub(4)GNSs (Fe sub(3)O sub(4)GNSs-PDA) was applied for Candida rugosa lipase (CRL) immobilization covalently without any toxic coupling agent. Combining the superior physical properties and chemical stability of Fe sub(3)O sub(4)GNSs and the well biocompatibility, functional characteristics of PDA, the Fe sub(3)O sub(4)GNSs-PDA composite displayed several advantages, including the high enzyme capacity, enzyme activity and stability and a decrease in enzyme loss. Our work demonstrated that the mussel-inspired Fe sub(3)O sub(4)GNSs can be extended to many other applications such as biocatalytic, genetic and industrial.
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