Journal Article

Response of extracellular carboxylic and thiol ligands (oxalate, thiol compounds) to Pb super(2+) stress in Phanerochaete chrysosporium

Huang, Danlian, Lai, Cui, Zeng, Guangming, Xu, Piao, Huang, Chao, Zhang, Chen, Yan, Ming, Cheng, Min, Li, Ningjie, Wei, Zhen

2015

Overview

When exposed to higher Pb super(2+) concentration, Phanerochaete chrysosporium secreted higher content of oxalate and thiol compounds. An earlier and faster increase in oxalate was observed after short-term exposure, comparing with a gentle increase in the thiol compounds. In the extracellular polymeric substances (EPS) extract, more oxalate and T-SH were detected when the initial Pb super(2+) was higher, and the variations were different from the situation in the culture medium. In EPS solution, the oxalate amount was more closely related with Pb than that of thiol compounds. pH condition in the whole Pb removal process by P. chrysosporium ranged from 4 to 6.5 and was more beneficial for the binding of Pb super(2+) to carboxylic groups in the oxalic acid. More Pb super(2+) induced more EPS amount, and the increase of EPS amount influenced the immobilized oxalate more seriously. The present study can supply more comprehensive information about the metal passivation mechanism in white-rot fungi and provide meaningful references for an enhanced removal of heavy metals.

• Subject: Phanerochaete chrysosporium