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Defects in inositol 1,4,5-trisphosphate receptor expression, Ca super(2+) signaling, and insulin secretion in the anx7(+/-) knockout mouse
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Defects in inositol 1,4,5-trisphosphate receptor expression, Ca super(2+) signaling, and insulin secretion in the anx7(+/-) knockout mouse
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Defects in inositol 1,4,5-trisphosphate receptor expression, Ca super(2+) signaling, and insulin secretion in the anx7(+/-) knockout mouse
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Journal Article
Defects in inositol 1,4,5-trisphosphate receptor expression, Ca super(2+) signaling, and insulin secretion in the anx7(+/-) knockout mouse
1999
Overview
The mammalian anx7 gene codes for a Ca super(2+)-activated GTPase, which supports Ca super(2+)/GTP-dependent secretion events and Ca super(2+) channel activities in vitro and in vivo. To test whether anx7 might be involved in Ca super(2+) signaling in secreting pancreatic beta cells, we knocked out the anx7 gene in the mouse and tested the insulin-secretory properties of the beta cells. The nullizygous anx7 (-/-) phenotype is lethal at embryonic day 10 because of cerebral hemorrhage. However, the heterozygous anx7 (+/-) mouse, although expressing only low levels of ANX7 protein, is viable and fertile. The anx7 (+/-) phenotype is associated with a substantial defect in insulin secretion, although the insulin content of the islets, is 8- to 10-fold higher in the mutants than in the normal littermate control. We infer from electrophysiological studies that both glucose-stimulated secretion and voltage-dependent Ca super(2+) channel functions are normal. However, electrooptical recordings indicate that the (+/-) mutation has caused a change in the ability of inositol 1,4,5-trisphosphate (IP sub(3))-generating agonists to release intracellular calcium. The principle molecular consequence of lower anx7 expression is a profound reduction in IP sub(3) receptor expression and function in pancreatic islets. The profound increase in islets, beta cell number, and size may be a means of compensating for less efficient insulin secretion by individual defective pancreatic beta cells. This is a direct demonstration of a connection between glucose-activated insulin secretion and Ca super(2+) signaling through IP sub(3)-sensitive Ca super(2+ )stores.
