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Xanthomonas euvesicatoria in pepper seeds: Implementation of its detection and preliminary Study on its genetic fingerprints
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Xanthomonas euvesicatoria in pepper seeds: Implementation of its detection and preliminary Study on its genetic fingerprints
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Xanthomonas euvesicatoria in pepper seeds: Implementation of its detection and preliminary Study on its genetic fingerprints
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Journal Article
Xanthomonas euvesicatoria in pepper seeds: Implementation of its detection and preliminary Study on its genetic fingerprints
2015
Overview
The bacterial spot of pepper is a destructive disease and its causal agent was formerly known as Xanthomonas campestris pv. vesicatoria, later reclassified in four different species: X. vesicatoria, X. euvesicatoria, X. perforans and X. gardneri. All four species are seed-borne and regulated. Xanthomonas euvesicatoria is particularly aggressive on pepper and its detection and identification, particularly In seed lots, is the key for a safe pepper production. We compared a conventional serological detection method (ELISA) with The direct isolation and identification of the pathogen, and with a specific molecular detection (simplex-PCR), but following two different DNA extraction and purification procedures in parallel:1) treatment of the seed concentrate by a heat shock or 2) use of the DNeasy Plant Mini Kit columns (Qiagen). Thirteen ELISA positive samples were found: from those seed samples, 5000 seeds for each extraction method were taken and extracted according the two procedures mentioned above. Results highlighted that ELISA performed quite well, but the most sensitive and reliable pathogen detection was done by seed extraction with the DNeasy Plant Mini Kit, followed by simplex-PCR. Direct isolation and simplex-PCR Following heat shock gave several false negative results. Genotyping of a X. euvesicatoria isolate collection was attempted through rep-PCR, using the BOX, REP and ERIC primers to specifically amplify repetitive elements dispersed throughout the bacterial genome.Results highlighted that X. euvesicatoria is quite a uniform population, taxonomically not so distant from X. perforans, but clearly distinguished from the other two xanthomonads. Analysis of BOX-PCR profiles supports the fact that a possible X. euvesicatoria subgroup is present in the main population, which might be Related to a different geographical origin of seeds.
