Journal Article

Protective effect of Raphanus sativus on H sub(2)O sub(2) induced oxidative damage in human lymphocytes

Mangamoori, Lakshmi Narasu, Reddy, Lingam Venkata, Beevi, Syed Sultan

2010

Overview

Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. This present study evaluated the protective effect of different parts of R. sativus such as root, stem and leaf obtained with a variety of extraction solvents against cell death and oxidative DNA damage induced by hydrogen peroxide (H sub(2)O sub(2)) in normal human lymphocytes. R. sativus extracts as such showed no cytotoxicity and genotoxicity to the lymphocytes at the tested concentrations. Of the different extracts, hexane extract of root and methanolic extract of stem and leaf showed significant protective effect against oxidative damage induced by 200kM H sub(2)O sub(2) in a dose dependent manner, as compared to cells exposed only to H sub(2)O sub(2). Our results suggest that the protective effect afforded by R. sativus extract could be related to the presence of isothiocyanates and polyphenolics, as they possess significant capacity to remove reactive species by virtue of their ability to scavenge free radicals and induce antioxidant enzyme system in the cells.

• Subject: Raphanus sativus