Journal Article

Factors Associated with Determination of Root super(22)Na super(+) Influx in the Salt Accumulation Halophyte Suaeda maritima

Wetson, Anne M, Gurmani, Ali R, Wang, Suo-Min, Wang, Chun-Mei, Zhang, Jin-Lin, Bao, Ai-Ke

2011

Overview

Salinity is an increasing problem for agricultural production worldwide. The result of low-affinity Na super(+) uptake is toxic to the cytoplasm of most crop plants. Nevertheless, the pathways for this low-affinity Na super(+) uptake are still uncertain. In this work we used super(22)Na super(+) isotope tracing technology to investigate factors associated with determination of root super(22)Na super(+) influx in the salt accumulation halophyte Suaeda maritima. We found that a 2min of exposure to the super(22)Na super(+) labeled uptake solution was optimal for determining super(22)Na super(+) influx into excised roots of S. maritima and that 7 min of blotting is suitable in super(22)Na super(+) influx experiments. super(22)Na super(+) influx did not increase linearly with the increasing external Na super(+) concentration, in the range tested, of 2 to 300mM NaCl. But root super(22)Na super(+) influx and root Na super(+) concentration were well correlated. super(22)Na super(+) influx into excised roots of S. maritima was not, however, well correlated with the plant size. All the above results indicated further that this super(22)Na super(+) isotope influx procedure is a good method for quantify Na super(+) uptake rate by the roots of the salt accumulation halophyte.

• Subject: Suaeda maritima