Asset Details
Do you wish to reserve the book?
Self-Crosslinking of Silk Fibroin Using H 2 O 2 -Horseradish Peroxidase System and the Characteristics of the Resulting Fibroin Membranes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Self-Crosslinking of Silk Fibroin Using H 2 O 2 -Horseradish Peroxidase System and the Characteristics of the Resulting Fibroin Membranes
Do you wish to request the book?
Self-Crosslinking of Silk Fibroin Using H 2 O 2 -Horseradish Peroxidase System and the Characteristics of the Resulting Fibroin Membranes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Self-Crosslinking of Silk Fibroin Using H 2 O 2 -Horseradish Peroxidase System and the Characteristics of the Resulting Fibroin Membranes
2017
Overview
Silk fibroin has been widely used in biomedical and clinical fields owing to its good biocompatibility. In the present work, self-crosslinking of fibroin molecules was carried out using the hydrogen peroxide (H
O
)-horseradish peroxidase system, followed by preparation of the fibroin membranes, aiming at improving the mechanical property of fibroin-based material and expanding its applications. P-Hydroxyphenylacetamide (PHAD), as the model compound of tyrosine residues in fibroins, was used to investigate the possibility of horseradish peroxidase (HRP)-catalyzed crosslinking. The results were characterized by means of 1H NMR and UPLC-TQD. The efficacy of enzymatic crosslinking of silk fibroins was examined by determining the changes in the relative viscosity, amino acid compositions, and SEC chromatogram. The obtained data indicated that H
O
-HRP incubation led to PHAD polymerization, and the molecular weight of fibroin proteins was also noticeably increased after the enzymatic treatment. CD and ATR-FTIR spectra revealed that H
O
-HRP treatments had an evident impact on the conformational structure of silk fibroins. The mechanical property and thermal behavior for the modified fibroin membrane were noticeably improved compared to the untreated. Meanwhile, the obtained membrane exhibited good biocompatibility according to the cell growth experiment. The present work provides a novel method for preparation of the fibroin-based materials for biomedical applications.
