Journal Article

Muscle Damage in Dystrophic mdx Mice Is Influenced by the Activity of Ca 2+ -Activated K Ca 3.1 Channels

Mozzetta, Chiara, Grassi, Francesca, Wulff, Heike, Capitani, Riccardo, Morotti, Marta, Cocozza, Germana, Bianconi, Valeria, Limatola, Cristina, De Stefano, Maria Egle, Catalano, Myriam, Garofalo, Stefano, Antonangeli, Fabrizio

2022

Overview

Duchenne muscular dystrophy (DMD) is an X-linked disease, caused by a mutant dystrophin gene, leading to muscle membrane instability, followed by muscle inflammation, infiltration of pro-inflammatory macrophages and fibrosis. The calcium-activated potassium channel type 3.1 (K 3.1) plays key roles in controlling both macrophage phenotype and fibroblast proliferation, two critical contributors to muscle damage. In this work, we demonstrate that pharmacological blockade of the channel in the mouse model during the early degenerative phase favors the acquisition of an anti-inflammatory phenotype by tissue macrophages and reduces collagen deposition in muscles, with a concomitant reduction of muscle damage. As already observed with other treatments, no improvement in muscle performance was observed in vivo. In conclusion, this work supports the idea that K 3.1 channels play a contributing role in controlling damage-causing cells in DMD. A more complete understanding of their function could lead to the identification of novel therapeutic approaches.