Journal Article

N -Feruloyl Serotonin Attenuates Neuronal Oxidative Stress and Apoptosis in Aβ 25-35 -Treated Human Neuroblastoma SH-SY5Y Cells

Kim, Jihyun, Cho, Eunju, Shin, Yusu, Park, Chanhum, He, Meitong

2023

Overview

Amyloid-beta (Aβ) aggregation and deposition have been identified as a critical feature in the pathology of Alzheimer's disease (AD), with a series of functional alterations including neuronal oxidative stress and apoptosis. -feruloyl serotonin (FS) is a plant-derived component that exerts antioxidant activity. This study investigated the protective effects of FS on Aβ -treated neuronal damage by regulation of oxidative stress and apoptosis in human neuroblastoma SH-SY5Y cells. The radical scavenging activities increased with the concentration of FS, exhibiting in vitro antioxidant activity. The Aβ -treated SH-SY5Y cells exerted neuronal cell injury by decreased cell viability and elevated reactive oxygen species, but that was recovered by FS treatment. In addition, treatment of FS increased anti-apoptotic factor B-cell lymphoma protein 2 (Bcl-2) and decreased the pro-apoptotic factor Bcl-2-associated X protein. The FS attenuated Aβ-stimulated neuronal apoptosis by regulations of mitogen-activated protein kinase signaling pathways. Moreover, activated CREB-BDNF signaling was observed by the treatment of FS in Aβ -induced SH-SY5Y cells. These results demonstrate that FS shows potential neuroprotective effects on Aβ -induced neuronal damage by attenuation of oxidative stress and apoptosis, and suggest that FS may be considered a promising candidate for the treatment of AD.

• Subject: Alzheimer Disease / Amyloid beta-Peptides - metabolism / Antioxidants - pharmacology / Apoptosis / Cell Line, Tumor / Humans / Neuroblastoma - pathology / Neuroprotective Agents - pharmacology / Oxidative Stress / Peptide Fragments - metabolism / Reactive Oxygen Species - metabolism / Serotonin - metabolism