Journal Article

Alleviating Clostridium perfringens -Induced Intestinal Lesions in Chickens Using the Xylanase Cb Xyn10C and Its Binary Cocktail with a Protease

Luo, Huiying, Guo, Kairui, Yao, Bin, Su, Xiaoyun, Sun, Xianhua, Wang, Yuan, Hao, Zhenzhen, Zhang, Wenjing, Wang, Shuai, Wang, Tong, Yang, Daoxin, Ji, Wangli, Yang, Shuyan, Zhang, Meiling, Huang, Huoqing, Ma, Jianshuang

2025

Overview

infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase ( Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of were analyzed for their ability to alleviate -induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no challenge; Cp, no enzyme, challenge; Xyn, Xyn10C plus challenge; Xyn+Am, Xyn10C+Amylase plus challenge; Xyn+Ap, Xyn10C+Alkaline protease plus challenge). The CVCC 60102 was administered orally on a daily basis to the chickens from 14 to 20 days. In comparison with Cp, Xyn+Ap significantly reduced intestinal damage in the duodenum, jejunum, and ileum of chickens challenged with ( < 0.05). The enzymes, and particularly Xyn+Ap, notably enhanced the expression of key intestinal barrier genes, reduced the IL-6 level, and decreased the DAO (diamine oxidase) level. Not unexpectedly, feeding enzymes influenced the abundance of and bacteria in the intestine. These results indicated that Xyn10C and protease can be used to alleviate intestinal damage caused by infection.