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电针下调内脏高敏感性大鼠结肠NGF和NGFR的表达
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Journal Article
电针下调内脏高敏感性大鼠结肠NGF和NGFR的表达
2015
Overview
R2-03; 目的：观察电针对内脏高敏感大鼠结肠神经生长因子(nerve growth factor, NGF)和神经生长因子受体(nerve growth factor receptor, NGFR)表达的影响，探讨电针治疗慢性内脏高敏感性的作用机制。方法：将24只新生乳鼠随机分为正常组、模型组和电针组。参照 Al-Chaer 法制备内脏高敏感性大鼠模型。电针组予电针天枢、上巨虚治疗，每次20分钟，每天1次，连续7天。首次治疗后，通过观察大鼠腹壁撤回反射(abdominal withdrawal reflex, AWR)评分以评价内脏高敏感性大鼠的痛阈。治疗7天后，采集大鼠结肠组织进行NGF和NGFR免疫组织化学检测。结果：模型组大鼠的AWR评分均高于正常组，经电针治疗后均降低。模型组大鼠的结肠NGF和NGFR阳性表达较正常组显著增加(P＜0.05)，电针组治疗后NGF和NGFR阳性表达均显著降低(P＜0.001)。结论：电针可使内脏高敏感性大鼠的痛阈升高，并降低结肠的NGF和NGFR表达。电针对结肠NGF和NGFR表达的调节可能是其治疗慢性内脏高敏感性的外周作用机制之一。
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