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研究了离体后毛竹笋纤维素、木质素含量以及苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）活性的变化。结果显示：笋体纤维素、木质素含量从笋尖到笋基部逐渐增加；PAL、POD活性笋尖明显高于笋基部。离体后贮藏的竹笋纤维素、木质素含量随贮藏时间的延长而增加，在前5d纤维素的增加速度极快，在后5d增加速度明显趋缓，但木质素含量随贮藏时间的延长基本呈匀速态势增加；POD、PAL活性随贮藏时间的延长，活性均显著增加。对离体竹笋进行低温处理（4℃）可显著降低竹笋的PAL、POD活性，减少木质素和纤维素合成，低温对纤维素的影响高于木质素。

利用小佛肚竹、凤尾竹和孝顺竹幼竹的茎尖和带节茎段研究了愈伤组织诱导和控制褐变的方法。试验结果表明：在3种生长调节剂（2，4-D、KT、IBA）的组合中，以2，4-D（2～3mg·L^-1）效果最好，愈伤组织的诱导率最高，大部分外植体都能诱导出愈伤组织，生长良好；2，4-D2mg·L^-1＋KT0．5mg·L^-1能使部分茎段诱导出愈伤组织，而茎尖诱导的愈伤组织水渍化，易褐变；KT4mg·L^-1能使个别茎段有愈伤组织产生，但易褐化；KT4mg·L^-1＋IBA0．5mg·L^-1能诱导出愈伤组织，但生长缓慢。添加抗氧化剂控制愈伤组织褐变的效果要优于吸附剂，其防褐化能力：抗坏血酸（5mg·L^-1）〉半胱氨酸（100mg·L^-1）〉PVP（1g·L^-1）〉活性炭（1g·L^-1）。在培养基中加入抗坏血酸（5mg·L^-1），使小佛肚竹、凤尾竹、孝顺竹的茎尖和茎段的褐变率分别比对照下降了51．3％、43．3％、36．8％和62．7％、42．7％、30．8％。外植体在无菌水或半胱氨酸（100mg·L^-1）溶液中浸泡2～4h也有利于控制褐化。暗培养有利于愈伤组织生长，对控制褐化也有一定作用。

The contents of cellulose, lignin and activities of POD, PAL in excised bamboo shoots of Phy llostachysedu lis were studied. The result showed that in excised bamboo shoots from top part to base part, the contents ofcellulose and lignin increased gradually, while the activities of POD and PAL on top part of shootwasmore than thatof base part. Along with p rolongation of the storage time, the contents of cellulose and lignin increase rap idly. Inthe first 5 days cellulose increase rap idly, in the second 5 days it becomes to slow down apparently. But the lignincontent increased in a even speed during the storage time. At the same time the activities of POD and PAL increasedsignificantly with the storage time. Bamboo shoots treated by low temperature (4 ℃) showed that low temperaturecould decrease the activities of POD and PAL significantly, aswell as decrease the contents of cellulose and ligninsignificantly. The influence of low temperature for cellulose was bigger than that for lignin. 研究了离体后毛竹笋纤维素、木质素含量以及苯丙

The stem tops and stems with node of young bamboo of Bambusa ventricosa,B. multiplex cv. Fernleaf and B. multiplex were used to study the callus induction and brown stain control. The results showed that among three regulator combinations(2,4-D KT and IBA) ,2,4-D(2-3 mg·L-1)treatment was the best with the highest callus induction rate,most of the explants induced callus and grew well. 2,4-D(2 mg·L-1) + KT(0. 5 mg·L-1) treatment could induce callus from stem parts while the callus induced from the stem top was water-soaked and easy to be brown stained. KT(4 mg·L-1) treatment could induce callus on some stems but was easy to be brown stained. KT(4 mg·L-1) + IBA(0. 5 mg·L-1) treatment could induce callus but they grew very slowly. The effect of adding anti-oxidant was better than adding adsorbent in controlling brown stain of callus. The ability of brown stain control is in the order of ascorbic acid(5 mg·L-1) > cyste-ine(100 mg·L-1) > activated carbon(1 g·L-1) . By adding ascorbic acid(5 mg·L-1) in culture medi