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为研究超干燥保存手段运用于坡柳种子的可行性，采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来分析。用硅胶将种子脱水至1．12％～6．13％6个含水量梯度，结合不同处理密封保存1年，随后测定发芽率和一些生理生化指标，包括相对电导率（RC）、脯氨酸（Pro）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、可溶性总糖含量和脂肪酸组分。结果表明：种子含水量是对发芽率影响最显著的因素，较为适宜的脱水范围为4．28％～5．25％；超干种子发芽率与相对电导率和丙二醛含量呈显著负相关，与脯氨酸含量和3种抗氧化酶活性呈显著正相关；超干种子的油酸和d．亚麻酸含量比对照提高。坡柳种子保存过程中的劣变是由细胞膜损伤和脂质过氧化所引起，而适宜的脱水处理能使抗氧化酶活性增强，使不饱和脂肪酸的含量增加，从而维持种子生活力的稳定。

以云南省境内金沙江、元江、红河和怒江流域自然分布的构树为试材，采用AFLP分子标记技术对90份构树种质资源进行了遗传多样性分析研究。结果表明：筛选出的7对引物组合共扩增获得786条清晰可辩的条带，其中，多态性带632条，多态性条带百分率达80．4％，平均每对引物组合检测出90．3个多态位点。分布于4条水系流域的构树居群间，金沙江流域构树居群的遗传多样性水平最高，Nei’s基因多样性指数为0．1455，而元汀流域构树居群的遗传多样性水平最低，Nei’s基因多样性指数为0．1129。4个构树居群间的遗传分化系数为0．0386，表明构树的遗传变异主要存在于居群内不同个体之间。在遗传距离为0．003时，4个构树居群可分为2组，第1组南金沙江流域的构树居群构成，第2组包含分布于红河、怒江和元江流域的3个构树居群。

Ultra-dry storage of Dodonaea viscose seeds was studied by orthogonal design including four factors (seed moisture content, packing method, storage temperature and pre-humidification method). Seeds were dried to the moisture contents ranging from 1.12% to 6.13% with silica gel, then stored in hermetic condition combined with different treatments for a year. The germination percentage, electrical conductivity (RC), proline (Pro), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total soluble sugar content and fatty acid composition of the stored seeds were tested. The results showed that the seed moisture content had significant effect on germination percentage. The feasible moisture content ranged from 4.28% to 5.25%.The Germination percentage had a significantly negative correlation with the electrical conductivity and proline content, and had significantly positive correlation with the proline content and activity of three antioxidant enzymes. The content of oleic acid an

Ninty Broussonetia papyrifera samples were collected from four river basins in Yunnan province and used as materials, and AFLP markers were employed to analyze their genetic diversity. The results showed that total 786 unambiguous bands were revealed from selected 7 primer combinations, of which 632 bands were polymorphic, and the ratio of polymorphic bands was 80.4 %. An average of 90.3 polymorphic bands was detected from each primer combination on average. Among four B. papyrifera populations, the population of Jinsha-river Basin held the highest level of genetic diversity, the Nei's gene diversity being 0.145 5, while the population of Yuanjiang-river Basin held the lowest level of genetic diversity, the Nei's gene diversity being 0.112 9. The genetic differentiation coefficient was 0.038 6, which indicated that the genetic variation of individuals within population being the main sources of genetic diversity in B. papyrifera. The four populations of B. papyrifera could be divided into two groups with the