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Abstract The Javaen barb Systomus rubripinnis is an endemic fish not domesticated in Indonesia. This study aims to evaluate the effectiveness of gonadotropin and melatonin hormones in inducing ovulation of female Javaen barb. A total of 12 female fish (BW: 142.12 ± 18.08 g; egg diameter 1.0-1.2 mm) were selected to be injected with a combination of different hormones. The treatments were ovaprimTM at a dose of 0.6 mL/kg without melatonin (L0.6M0), ovaprimTM at 0.3 mL/kg with 0.25 mg/kg melatonin (L0.3M0.25), 500 IU/kg of human chorionic gonadotropin with 0.25 mg/kg melatonin (H500M0.25), and 0.25 mg/kg melatonin (M0.25). The L0.6M0 and L0.3M0.25 treatments were injected twice. The first injection was 40% of the total dose, while the rest (60%) was injected 6 hours after the first injection. Melatonin injection was carried out at the same time as the first injection. In the H500M0.25 treatment, melatonin injection was carried out 24 hours after HCG injection. Fish injected with ovaprimTM with and without melatonin had the fastest latency period, and ovulation occurred in all fish. The H500M0.25 treatment had an ovulation rate of 66.7%, while those injected with only melatonin (M0.25) did not ovulate. The number of ovulated eggs, fertilization and hatching rate from ovaprimTM injected broodstock were higher than those of HCG. In contrast, the larvae’s survival rate, body weight, and length were similar. In conclusion, ovaprimTM is practical in inducing ovulation of Javaen barb, and melatonin has a complementary effect on Javaen barb ovulation. Resumo O barbo de Java Systomus rubripinnis é um peixe endêmico não domesticado na Indonésia. Este estudo teve como objetivo avaliar a eficácia dos hormônios gonadotrofina e melatonina na indução da ovulação de fêmeas de barbo de Java. Um total de 12 fêmeas (PC: 142,12 ± 18,08 g; diâmetro do ovo 1,0-1,2 mm) foi selecionado para receber uma combinação de diferentes hormônios. Os tratamentos foram ovaprimTM na dose de 0,6 mL/kg sem melatonina (L0,6M0), ovaprimTM a 0,3 mL/kg com 0,25 mg/kg de melatonina (L0,3M0,25), 500 UI/kg de gonadotrofina coriônica humana com 0,25 mg/kg de melatonina (H500M0,25) e 0,25 mg/kg de melatonina (M0,25). Os tratamentos L0,6M0 e L0,3M0,25 foram injetados duas vezes. A primeira injeção foi de 40% da dose total, enquanto o restante (60%) foi injetado 6 horas após a primeira injeção. A injeção de melatonina foi realizada ao mesmo tempo que a primeira injeção. No tratamento H500M0,25, a injeção de melatonina foi realizada 24 horas após a injeção de HCG. Peixes injetados com ovaprimTM com e sem melatonina apresentaram o período de latência mais rápido, e a ovulação ocorreu em todos os peixes. O tratamento H500M0,25 apresentou uma taxa de ovulação de 66,7%, enquanto aqueles injetados apenas com melatonina (M0,25) não ovularam. O número de ovos ovulados, a fertilização e a taxa de eclosão dos reprodutores injetados com ovaprimTM foram maiores do que os do HCG. Em contraste, a taxa de sobrevivência, o peso corporal e o comprimento das larvas foram semelhantes. Em conclusão, o ovaprimTM é prático na indução da ovulação do barbo de Java e a melatonina tem um efeito complementar na ovulação do barbo de Java.

This study was aimed to optimize the spermatozoa cryopreservation protocol in Albino pangasius catfish by assessing the effectiveness of different cryoprotectant concentrations and different time of equilibration. The first experiment was conducted to optimize the combination of 10% methanol with different concentration of skim milk (0%, 5%, 10%, 15%, and 20%). The second experiment was aimed to optimize different equilibration time (10, 20, and 30 minutes). Deep freezer was used for the cryopreservation with a temperature of -80 °C at a storage period of 8 days. The first study found that the viability and motility of sperm after cryopreservation was significantly different (P<0.05) with fresh sperm. The best result was obtained at the 10% skim milk concentration with sperm viability of 78 ± 4.35% and motility of 69.66 ± 1.52%. The second experiment identified a significant different (P<0.05) in sperm viability and motility between fresh sperm and sperm post equilibration. The best result was obtained at 10 minutes equilibration time with sperm viability of 76 ± 1.15% and motility of 66 ± 2.64%. The study concluded that the 10% methanol with 10% skim milk, and 10 minutes equilibration time is the best combination for cryopreservation of Albino pangasius catfish spermatozoa.

Handling Dengue Hemorrhagic Fever (DHF) becomes Indonesia's national priority. DHF is an infectious disease whose treatment requires precision and speed of diagnosis. Data mining can be used to build prediction diagnosing DHF disease with supporting database. This paper aims to predict DHF using SVM, Naive Bayes, and Random Forest and then compare it with the accuracy of the result of third method. The accurate DHF prediction system can be used to avoid the error of diagnosing DHF and the treatment of the disorder can be done more quickly and precisely. The input systems are the patient's medical records (i.e. temperature, spotting, rumple leed, and bleeding) and the output system is suffering from DHF or not.

The broiler is one of the chicken to be able to grow fast for producing meat in a relatively short time. In modern times broiler farms have entered the industrial scale. Poultry in this case broiler chickens breathe by inhaling oxygen and releasing carbon dioxide gas and water. In this case, if the oxygen content in the cage is too low, this condition is related to the temperature and humidity of the cage, it will affect the production and health of the chicken. This study aims to determine to find out the distribution of carbon dioxide in broiler farming in a closed house. Method, Equipment was used namely hardware and software specifications; the first, Laptop Creation of programs and real-time data retrieval is done using an Intel Core i3 - 2310M type laptop, 2 GB RAM memory and Microsoft Windows 7 operating system. The second, the Toolbox. power, driver, actuator. The three Closed House and Enclosure Support Devices measuring 1.8m x 1.3 m x 1m are made of boards, equipped with fans, lights, chickens, and sensors. The research support tools include: multimeters, cables, solder, lead, and lead suction. The fifth, MATLAB Software Th system designed is simulated with MATLAB software version 7.8.0.347 (R2009a) with Simulink facilities. The sixth is Code Vision AVR software for creating and coding programs. Code vision used is version 1.25.9. The results of monitoring system carbon dioxide test integrated in real-time the first produce 500 ppm in free air while the second test produces 1000 ppm carbon dioxide if based on the characteristics still carbon dioxide threshold in the closed house.

Dangke ripening cheese is a dangke cheese that is processed by ripening with lactic acid bacteria to improve the taste, self-life of the product, and diversify it with new flavors. In this study, consumer tests were carried out after the packaging process and product self-life test at cold storage, room temperature, and freezing temperature, to see the product expiry period. The parameters measured were consumer acceptance, physical, chemical, and microbiological quality. The results showed that the physical characteristics of Dangke ripening with L. lactis inoculation and packaged with banana leaves and vacuum plastic were the best quality when stored at refrigerator temperatures and still not damaged until storage for 15 days, while at room temperature storage has shown a decrease quality. Frozen storage indicated that physical quality was still good for 15 days but tends to be less favored by consumers, possibly because the texture has changed.

In order to lower the fish mortality during transportation, the chopped banana stem (BS) was added during the African catfish transport procedure. The fish were packed in plastic bags with a density of 100 fish in 1.5 L water. The chopped BS was added into the plastic bags with different concentrations: 0 (control), 5, 10, and 15 mg L −1 . The fish were transported for 5 hours. After transportation, the fish, together with the added chopped BS, were distributed into glass tanks and acclimated for 24 h. After 24 h, the results showed that the BS treatments had lower cumulative mortality compared to the control with the lowest mortality was observed at the 15 mg L −1 concentration (p<0.05). The BS treatments also increased the antioxidant defence and the immune status of the fish thus might be responsible for the reduced mortality.

Geodatabase or digital spatial database is one of the GIS applications with an integrated database that has become a data source center that can be accessed for information and analysis needs by various existing applications. Participatory digital database has the potency to be used for inventorizing and collecting a complete, comprehensive, and detailed village/sub-district potentials spatial data. This research aims to provide knowledge as well as skills to the community, especially sub-district officials about a standardized spatial-based digital database of village potentials. The study took place in Langnga village, Mattiro Sompe District, Pinrang Regency, South Sulawesi, Indonesia. Methods used in this research are UAV Data Processing, Geodatabase System Compilation, Census and Cadastre Surveys, and Integration of Non-Spatial Data with Spatial Data. By compiling village potentials geodatabases, we constructed a Langnga village’s potentials map which can be used as a reference for planning and development of the village.

Village maps can provide geospatial data and information that would enable them to become an important instrument in regional development planning. The purpose of this study was to produce geospatial data and information on coastal village resources based on the Indonesian Geographic Information Agency (BIG) reference guidelines. The study was conducted in Langnga Village, Mattiro Sompe District, Pinrang Regency, South Sulawesi Province, Indonesia. This research used raster and vector data. Raster data consisted of high-resolution images obtained from Unmanned Areal Vehicles. The ground truthing used open access applications and interviews with the community. The licensed Arc.GIS 10.5 software was used for the spatial analysis. The dominant land cover/land use categories were aquaculture ponds and rice fields. These areas have historically undergone a dynamic change of land use from rice fields to ponds and back again. Other land uses were settlements inhabited by farmers and fishermen. White sand beaches are one potential resource in this villages, but they are prone to abrasion. The spatial information contained in the village resource and boundary map can be considered as a breakthrough step and could become a pilot for other villages to follow.

Migrant children and adolescents face a significantly increased risk of mental health issues. Focusing on this population's mental health issues is fundamental and requires more attention to detect and reduce these burdens in adulthood. Nevertheless, life skills intervention can improve mental health. Its effects on Arab migrant adolescents have not been tested. Here, an evaluation protocol of the effect of an online life skills-based intervention for improving depression, anxiety, stress, self-efficacy, and coping skills among Arab adolescents in Malaysia will be examined. This cluster randomised controlled trial (RCT) will involve 207 Arab students (14-18 years old) from 12 Arabic schools in the Klang Valley. The schools will be assigned randomly to an intervention (online life skills programme) or control group at a 1:1 ratio. The researcher will deliver eight one-hour sessions to the intervention group weekly. The control group will receive the intervention at the evaluation end. Both groups will complete assessments at baseline, and immediately and three months after the intervention. The primary outcome is anxiety, depression, and stress [Depression Anxiety and Stress Scale-21 (DASS-21)]. The secondary outcomes are self-efficacy (General Self-Efficacy Scale) and coping skills (Brief COPE Inventory). Data analysis will involve the Generalised Estimation Equation with a 95% confidence interval. P < .05 will indicate significant inter- and intra-group differences. This will be the first cluster RCT of an online life skills education programme involving Arab adolescent migrants in Malaysia. The results could support programme effectiveness for improving the participants' mental health problems (depression, anxiety, stress), increasing their self-efficacy, and enhancing their coping skills. The evidence could transform approaches for ameliorating migrant children and adolescents' mental well-being. The study is registered with the Clinical Trial Registry (Identifier: NCT05370443).

Aquaculture of the African catfish Clarias gariepinus has rapidly increased in South-East Asia over recent years, which has now become one of the most cultivated species. This culture intensification has resulted in the development of various diseases, among which the Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The present study aimed at investigating the potential of three plants, Piper betle, Psidium guajava, and Tithonia diversifolia, to prevent this disease using an enriched fish diet during 4 weeks. Though the minimum inhibitory and bactericidal concentrations of acetone–extracts were relatively high (2–32 mg mL−1 and 8–64 mg mL−1, respectively), all three plant powders imbedded in feed significantly reduced fish mortalities following an experimental infection with A. hydrophila, as indicated by relative percent survivals (RPS) ranging from 77 to 79%. When used in combination, the three plant extracts showed no interaction in vitro. Even though they improved RPS in comparison with controls, they were, however, less efficient in reducing mortalities than single plants. None of the plant-enriched diet had a clear effect on blood cell count or on reactive oxygen species (ROS) production (except for P. betle, which significantly increased ROS production after infection); however, both individual plants and their combinations significantly limited the drop of hematocrit following infection. Altogether, these results indicate that selection of plants for herbal therapy is complex and might depend on several in vivo criteria, and as such should not solely rely upon measurement of their antimicrobial activity.