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The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)
The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)
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The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)
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The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)
The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)

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The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)
The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)
Journal Article

The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842)

2025
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Overview
Abstract The Javaen barb Systomus rubripinnis is an endemic fish not domesticated in Indonesia. This study aims to evaluate the effectiveness of gonadotropin and melatonin hormones in inducing ovulation of female Javaen barb. A total of 12 female fish (BW: 142.12 ± 18.08 g; egg diameter 1.0-1.2 mm) were selected to be injected with a combination of different hormones. The treatments were ovaprimTM at a dose of 0.6 mL/kg without melatonin (L0.6M0), ovaprimTM at 0.3 mL/kg with 0.25 mg/kg melatonin (L0.3M0.25), 500 IU/kg of human chorionic gonadotropin with 0.25 mg/kg melatonin (H500M0.25), and 0.25 mg/kg melatonin (M0.25). The L0.6M0 and L0.3M0.25 treatments were injected twice. The first injection was 40% of the total dose, while the rest (60%) was injected 6 hours after the first injection. Melatonin injection was carried out at the same time as the first injection. In the H500M0.25 treatment, melatonin injection was carried out 24 hours after HCG injection. Fish injected with ovaprimTM with and without melatonin had the fastest latency period, and ovulation occurred in all fish. The H500M0.25 treatment had an ovulation rate of 66.7%, while those injected with only melatonin (M0.25) did not ovulate. The number of ovulated eggs, fertilization and hatching rate from ovaprimTM injected broodstock were higher than those of HCG. In contrast, the larvae’s survival rate, body weight, and length were similar. In conclusion, ovaprimTM is practical in inducing ovulation of Javaen barb, and melatonin has a complementary effect on Javaen barb ovulation. Resumo O barbo de Java Systomus rubripinnis é um peixe endêmico não domesticado na Indonésia. Este estudo teve como objetivo avaliar a eficácia dos hormônios gonadotrofina e melatonina na indução da ovulação de fêmeas de barbo de Java. Um total de 12 fêmeas (PC: 142,12 ± 18,08 g; diâmetro do ovo 1,0-1,2 mm) foi selecionado para receber uma combinação de diferentes hormônios. Os tratamentos foram ovaprimTM na dose de 0,6 mL/kg sem melatonina (L0,6M0), ovaprimTM a 0,3 mL/kg com 0,25 mg/kg de melatonina (L0,3M0,25), 500 UI/kg de gonadotrofina coriônica humana com 0,25 mg/kg de melatonina (H500M0,25) e 0,25 mg/kg de melatonina (M0,25). Os tratamentos L0,6M0 e L0,3M0,25 foram injetados duas vezes. A primeira injeção foi de 40% da dose total, enquanto o restante (60%) foi injetado 6 horas após a primeira injeção. A injeção de melatonina foi realizada ao mesmo tempo que a primeira injeção. No tratamento H500M0,25, a injeção de melatonina foi realizada 24 horas após a injeção de HCG. Peixes injetados com ovaprimTM com e sem melatonina apresentaram o período de latência mais rápido, e a ovulação ocorreu em todos os peixes. O tratamento H500M0,25 apresentou uma taxa de ovulação de 66,7%, enquanto aqueles injetados apenas com melatonina (M0,25) não ovularam. O número de ovos ovulados, a fertilização e a taxa de eclosão dos reprodutores injetados com ovaprimTM foram maiores do que os do HCG. Em contraste, a taxa de sobrevivência, o peso corporal e o comprimento das larvas foram semelhantes. Em conclusão, o ovaprimTM é prático na indução da ovulação do barbo de Java e a melatonina tem um efeito complementar na ovulação do barbo de Java.