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Very-long-chain fatty acids (VLCFA) are precursors for various lipids playing important physiological and structural roles in plants. Throughout plant tissues, VLCFA are present in multiple lipid classes essential for membrane homeostasis, and also stored in triacylglycerols. VLCFA and their derivatives are also highly abundant in lipid barriers, such as cuticular waxes in aerial epidermal cells and suberin monomers in roots. VLCFA are produced by the fatty acid elongase (FAE), which is an integral endoplasmic reticulum membrane multi-enzymatic complex consisting of four core enzymes. The 3-ketoacyl-CoA synthase (KCS) catalyzes the first reaction of the elongation and determines the chain-length substrate specificity of each elongation cycle, whereas the other three enzymes have broad substrate specificities and are shared by all FAE complexes. Consistent with the co-existence of multiple FAE complexes, performing sequential and/or parallel reactions to produce the broad chain-length-range of VLCFA found in plants, twenty-one KCS genes have been identified in the genome of Arabidopsis thaliana . Using CRISPR-Cas9 technology, we established an expression platform to reconstitute the different Arabidopsis FAE complexes in yeast. The VLCFA produced in these yeast strains were analyzed in detail to characterize the substrate specificity of all KCS candidates. Additionally, Arabidopsis candidate proteins were transiently expressed in Nicotiana benthamiana leaves to explore their activity and localization in planta . This work sheds light on the genetic and biochemical redundancy of fatty acid elongation in plants.

• Plant architecture is central in determining crop yield. In the short-day species strawberry, a crop vegetatively propagated by daughter-plants produced by stolons, fruit yield is further dependent on the trade-off between sexual reproduction (fruits) and asexual reproduction (daughter-plants). Both are largely dependent on meristem identity, which establishes the development of branches, stolons and inflorescences. • Floral initiation and plant architecture are modulated by the balance between two related proteins, FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1). We explored in woodland strawberry the role of the uncharacterised FveFT2 and FveFT3 genes and of the floral repressor FveTFL1 through gene expression analyses, grafting and genetic transformation (overexpression and gene editing). • We demonstrate the unusual properties of these genes. FveFT2 is a nonphotoperiodic florigen permitting short-day (SD) flowering and FveTFL1 is the long-hypothesised long-day systemic antiflorigen that contributes, together with FveFT2, to the photoperiodic regulation of flowering. We additionally show that FveFT3 is not a florigen but promotes plant branching when overexpressed, that is likely to be through changing axillary meristem fate, therefore resulting in a 3.5-fold increase in fruit yield at the expense of stolons. • We show that our findings can be translated into improvement of cultivated strawberry in which FveFT2 overexpression significantly accelerates flowering.

The Annual Conference embodied the leadership spirit of AAACN, bringing 1,026 registrants and more than 20 exhibitors together for a powerful hybrid program championed by insights from more than 75 speakers over a 5-day event held April 11 -15 at the Omni Orlando Resort at ChampionsGate in Orlando, FL. o Nearly 40 sessions addressed a variety of current issues and topics, including building a value-based care environment for successful outcome measures, creating ambulatory care telephone triage clinical educator roles to address clinical knowledge gaps, and recognizing the advocacy power of ambulatory care nurses - and preparing them to engage in health policy advocacy at the local, state, and national levels. o Outstanding poster presentations, including leadership and telehealth themed posters, as well as diverse clinical posters, offered mindful practice improvements, innovative strategies for patient care, and professional development pathways. o This year's hybrid program continued AAACN's skillful optimization of livestreamed sessions, convenient on-demand content, live forum chats, and the popular Leaderboard Challenge, with many registrants earning prizes. Calling for AAACN members to be active in providing service and leadership, Dr. Rosa shared a heartfelt plea for the specialty of ambulatory care nursing to leverage its voice to work toward justice in vital healthcare areas, from supporting palliative and end-of-life care equity to identifying and tackling social determinants of health inequities, including poverty. Topics supported discussion of workplace violence prevention efforts, with a focus on development of action planning and strategic response. o The AAACN Workplace Safety Position Statement, featured during the Town Hall, reinforced the association's stance of supporting practices that promote physically and psychologically safe healthcare settings, and encouraging AAACN members to demand safe and secure care environments for themselves and their patients, act as advocates in implementing policies and practices to improve nurse and patient safety, and utilize tools and interdisciplinary resources to promote safety. o The insightful discussion included support for development of ambulatory care nurse support to de-escalate and manage verbal violence via the telephone, recognition and management of cyber incivility, creating a zero tolerance to violence through training, education, and resources, as well as development of clinic-specific safety action plans to secure and protect ambulatory staff members. Validating, mobilizing, and furthering the nursing discipline as it relates to health promotion across the lifespan, equitable care across populations, and the realization of authentic value will create the power we need to propel patients to health,\" Start shared.

An event devoted to celebrating a nursing community stressed by the relentless performance demands of a global pandemic. 2022, hosted at the Westgate Las Vegas. o Nearly 50 sessions on topics including developing accurate, reliable processes to measure work activities of primary care nurses to the fundamentals of nurse-sensitive indicators and strategies for preventing burnout and promoting well-being, the conference spoke to vital areas of ambulatory care nurse experiences. o Superior poster presentations, including leadership and telehealth themed posters, as well as care coordination and transition management (CCTM(r)) and clinical posters, offered practice improvements, innovative strategies for patient care, and professional development pathways. o With the virtual component structured by AAACN leaders and staff during the height of the pandemic, the hybrid event skillfully optimized live-streamed sessions, convenient on-demand content posted in AAACN's Online Library, as well as live forum chats and the resurgence of AAACN's popular Leaderboard Challenge, with many registrants earning prizes. In concert with Martinez, health care leader, accomplished innovator and nursing champion Beverly Malone, PhD, RN, FAAN, shared expertise from her leadership role at the National League of Nurses in advancing the science of nursing education through promoting of greater collaboration among stakeholders, increasing diversity in nursing and nursing education, and advancing excellence in care for patients.

In strawberry, the axillary bud (AXB) can produce either an elongated stem called stolon giving a daughter-plant (asexual reproduction) or an inflorescence-bearing branch crown (BC) (sexual reproduction). The fate of the AXB depends on node position on the axis and on genetic and environmental factors. Here, in Fragaria vesca, we addressed the largely unanswered question of how molecular factors determine AXB fate. To get insights into the mechanisms already at play in a morphologically indistinguishable (undifferentiated) AXB, depending on its fate, we combined (1) the phenotypic characterization of AXB development throughout plant growth with (2) the RNA-seq analysis of undifferentiated AXBs, using three different genotypes producing either BCs or stolons (fvetfl1 and fvega20ox mutants, FveFT3 overexpressor). Results allowed the identification of genes regulating AXB fate and outgrowth, among which FveBRC1. The analysis of FveBRC1 expression in genotypes combining various traits (perpetual/seasonal flowering; runnering/runneless) and the generation of CRISPR/Cas9 brc1 mutants further demonstrated that FveBRC1 plays a central role in the determination of AXB fate in strawberry, in addition to its well-known function in BC outgrowth. These original results provide new insights into the determination of AXB fate and, consequently, the control of fruit yield in strawberry.

The thick cuticle covering and embedding epidermal cells of tomato (Solanum lycopersicum) fruit, a model for cuticle studies, plays important roles in fruit protection and quality. To further our understanding of the influence of cuticle components on cuticle architecture and properties, the next step is to engineer cuticle composition. Ideally, to avoid indirect effects at whole plant level, a fruit and epidermis-specific promoter should be used. Here, to overexpress and edit target genes in tomato, we selected a non-specific Lipid Transfer Protein 2 promoter (pronsLTP) with preferential activity in fruit epidermis, as shown by NLS/GFP fluorescence analysis. Overexpression of SlMYB75 driven by pronsLTP induced anthocyanin accumulation specifically in the growing fruit epidermis, consistent with the cuticle deposition pattern. In keeping with changes in anthocyanins, the cuticle composition in flavonoids and phenolic acids was altered, as shown by Raman microspectroscopy, as were several cuticle properties. We then edited the carotenoid PSY1 gene with a pronsLTP driven CRISPR/Cas9 system. Different PSY1 knockout alleles were detected predominantly in fruit exocarp and mesocarp of color-impaired pronsLTP::Cas9-Psy1 mutants, and very little in the leaf. A mutant carrying a heritable knock-out mutation in both fruit and leaf was also detected, indicating that mutants obtained with pronsLTP may require screening before being studied. Altogether, our results indicate that pronsLTP2 can efficiently result in gene overexpression and editing in fruit epidermis. Implications of these findings are important for the functional analysis and genetic engineering of cuticle-related genes in tomato.Competing Interest StatementThe authors have declared no competing interest.

\"The automation of the Coilmaster Jr. has bolstered our performance of long-run projects and, in doing so, saved us time and manpower,\" [Randy Sapko] reports. \"We do everything from graphic design to color printing to offset printing and wide-format production, so when it comes to the efficiency of our binding capabilities we require they are state-of-the-art and capable of fast turnaround. We secured this objective by incorporating the Coilmaster Jr. into our productions.\"

In November 2009, [Bryan Wheeler] oversaw the implementation of a Baum AutoSet 2020 folder in his Covington, Ky.-based operation. It replaced the existing manual setup Baum 2020 right angle folder as the workhorse folder for all production. Though Wheeler says the reasons for introducing the Baum AutoSet 2020 into Fidelity's production are \"too numerous to list\" he is quick to itemize the attributes that make Fidelity is newly welcomed Baum invaluable.

With today's on-demand digital printing systems, diverse digital file delivery options, and advanced software applications targeting the task of the prototype creation, insight is now more than ever the critical and very human component of the fine art of prototype delivery. Rob Wallace, managing partner of New York-based Wallace Church, is on the front lines of taking a package concept to maturity - from the glimmering imagination crafted by zealous marketing professionals, to a carefully designed concept that can stand on its own from a production standpoint. Like many leading package designers, Wallace Church acts as the liaison between concept and reality.