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Some antibiotics have lost their efficacy over common infections and this has led to the search for new antibiotics and chemically altering existing ones for a better control of infectious diseases. In the present study, Pyrenacantha grandiflora tubers extracts were conjugated with ampicillin, penicillin, vancomycin and silver nanoparticles and their antimicrobial activity was evaluated against Escherichia coli , Staphylococcus aureus and Klebsiella Pneumoniae . The reactions were confirmed by formation of new functional groups that were identified by Fourier transmission infrared spectroscopy (FTIR). Minimum inhibitory concentrations were determined using the microdilution assay. Minimum bactericidal concentrations and the fractional inhibition concentration index were also determined. FTIR analysis indicated different functional group associated with conjugation. The activity of ampicillin was improved when conjugated with silver nanoparticles against K. pneumonia and E. coli . Vancomycin showed improvement of activity when conjugated to silver nanoparticles against K. pneumonia . Penicillin was improved by acetone extracts and vancomycin showed to be more effective when conjugated with silver nanoparticles and water extracts. The conjugation of P. grandiflora with penicillin, ampicillin and vancomycin in the presence of silver nanoparticles improved their biological activities. Therefore, the conjugates are medicinally important and can be used to improve the activity of existing antibiotics.

Green synthesis using plant extracts has emerged as an eco-friendly, clean, and viable alternative to chemical and physical approaches. Herein, the leaf, stem, and root extracts of Lannea discolor were utilized as a reducing and stabilizing agent in synthesizing gold (AuNPs) and copper (CuNPs) nanoparticles. The formation of AuNPs and CuNPs, confirmed by their color change, was characterized by UV-Vis spectroscopy (UV-Vis), scanning electron microscopy analysis, and energy-dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), coupled with minimum inhibitory concentration (MIC) antibacterial assays. Gold nanoflowers (AuNFs), NPs, and CuNPs peaked at wavelengths of 316, 544, and 564 nm, respectively. TEM showed unexpected nanoflowers (30–97 nm) in the leaf extracts and spherical NPs (10–33 nm; 9.3–37.5) from stem and root extracts, while spherical CuNPs (20–104 nm) were observed from all the extracts. EDX confirmed the presence of metal salts, and FTIR revealed stable capping agents. AuNPs and NFs from L. discolor extracts showed appreciable antibacterial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), and Bacillus subtilis (ATCC 6633) when compared to the plant extracts. At the same time, none was observed from the CuNPs. These AuNPs and CuNPs are particularly appealing in various biomedical and conductivity manufacturing applications due to their shapes and sizes and economical and environmentally friendly production. To our knowledge, this is the first study of the synthesis of gold and copper nanoparticles from L. discolor.

Due to the emergence of drug-resistant microorganisms, the search for broad-spectrum antimicrobial compounds has become extremely crucial. Natural sources like plants and soils have been explored for diverse metabolites with antimicrobial properties. This study aimed to identify microorganisms from agricultural soils exhibiting antimicrobial effects against known human pathogens, and to highlight the chemical space of the responsible compounds through the computational metabolomics-based bioprospecting approach. Herein, bacteria were extracted from soil samples and their antimicrobial potential was measured via the agar well diffusion method. Methanolic extracts from the active bacteria were analyzed using the liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) technique, and the subsequent data was further analyzed through molecular networking approach which aided in identification of potential anti-microbial compounds. Furthermore, 16S rRNA gene sequencing enabled identification of the active bacterial isolates, where isolate 1 and 2 were identified as strains of Bacillus pumilus , whilst isolate 3 was found to be Bacillus subtilis . Interestingly, isolate 3 ( Bacillus subtilis ) displayed wide-ranging antimicrobial activity against the tested human pathogens. Molecular networking revealed the presence of Diketopiperazine compounds such as cyclo (D-Pro-D-Leu), cyclo (L-Tyr-L-Pro), cyclo (L-Pro-D-Phe), and cyclo (L-Pro-L-Val), alongside Surfactin C, Surfactin B, Pumilacidin E, and Isarrin D in the Bacillus strains as the main anti-microbial compounds. The application of the molecular networking approach represents an innovation in the field of bio-guided bioprospection of microorganisms and has proved to be an effective and feasible towards unearthing potent antimicrobial compounds. Additionally, the (computational metabolomics-based) approach accelerates the discovery of bioactive compounds and isolation of strains which offer a promising avenue for discovering new clinical antimicrobials. Finally, soil microbial flora could serve an alternative source of anti-microbial compounds which can assist in the fight against emergence of multi-drug resistance bacterial pathogens.

Prokaryotic organisms rely on a limited array of metabolites for survival, which varies according to their natural environment. For example, soil‐borne bacteria produce diverse metabolites, such as antibiotics, to thrive in their competitive surroundings, inhibiting the growth of nearby competing bacteria. The structural diversity of these compounds offers great analytical challenges, since there is no universal acquisition setting that can be applied to achieve their comprehensive coverage. Therefore, the use of a single experimental setup inevitably hinders the comprehensive metabolite coverage, which would affect the outputs. To address this, we propose employing a design of experiment (DoE) approach through the central composite design (CCD) to enhance the metabolite detection and broaden the coverage of the data‐dependent acquisition (DDA) mode of the UHPLC‐qTOF‐MS technique. Our study reveals that altering collision energy significantly enhances metabolite coverage compared to adjusting the DDA threshold of detection. Furthermore, the ability of global natural product social (GNPS)–based molecular network models to annotate metabolites is greatly influenced by data acquisition settings, particularly affecting MS 2 data. Interestingly, molecular networks constructed from averaged spectral data obtained through randomly selected DDA settings outperform those generated using customized settings through DoE modeling. This study demonstrates that in untargeted LC‐MS metabolomics, both collision energy and intensity threshold independently enhance metabolite coverage in untargeted metabolomics. However, their combined use results in even greater coverage. Consequently, we recommend adopting group‐based optimization over single‐point optimization for more comprehensive metabolite coverage and in‐depth exploration. However, caution should be taken in order to balance between robust data and redundancy.

This study focuses on time-dependent microwave-assisted biosynthesis of silver nanoparticles using aqueous peel extracts of Citrus paradisi as a reducing, stabilizing and capping agent with emphasis on its antibacterial property. Optical, structural and morphological properties of the synthesized Citrus paradisi peel extract silver nanoparticle (CPAgNp) were characterized using UV-visible spectrophotometer, transmission electron microscope (TEM), scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET) and X-ray diffractometer (XRD). The antimicrobial activity was evaluated using the well- and disc-diffusion methods. Characteristic surface plasmon resonance (SPR) wavelength in the range of 420-440 nm at an optimized intensity growth rate typical of silver nanoparticles was obtained. The synthesized CPAgNp are spherical in shape having the size range of 14.84 ± 5 nm and possessing fcc unit phase structure. The Citrus paradisi peel extract mediated AgNp were found to possess a broad-spectrum antimicrobial activity against water-borne pathogenic microbes.

Enteric diseases remain a serious health problem globally. High prevalence is evident in regions with poor socioeconomic conditions, poor sanitation, and inadequate clean water supply, such as South Africa. Designing an effective strategy, however, requires local knowledge, which can be particularly challenging to acquire in low-and middle-income countries. As the first step in this process, we investigated the prevalence and distribution of protozoan parasites and in the rural and urban gastrointestinal clinics of South Africa. A cross-sectional study was conducted to assess the prevalence of enteric parasites and in rural and urban communities of South Africa. Stool samples were collected from November 2013 to June 2015 from patients with diarrhea (n=227) and without diarrhea (n=257). DNA was extracted and a diagnostic Taqman qPCR assay was performed to detect these protozoan parasites, which was further confirmed by the Sanger sequencing of a few samples. Of the 484 stool specimens collected, 34% (166/484) were positive for either spp. or parasites, with only 5% containing both parasites (22/484). In both study populations, was the most prevalent parasite (overall 25%) followed by (19%). This study discovered that both and parasites might contribute to diarrheal disease in South Africa and are more prevalent in rural communities. Future studies are needed to identify the source of the infection and design appropriate interventions to reduce the burden of the disease. Enterik hastalıklar küresel olarak ciddi bir sağlık sorunu olmaya devam etmektedir. Güney Afrika gibi düşük sosyo-ekonomik koşulların, kötü sanitasyonun ve yetersiz temiz su kaynaklarının olduğu bölgelerde yüksek prevalans görülmektedir. Ancak etkili bir strateji tasarlamak için, düşük ve orta gelirli ülkelerde edinilmesi özellikle zor olabilecek yerel bir bilgi gerektirmektedir. Bu süreçte biz ilk adım olarak, Güney Afrika’nın kırsal ve kentsel gastrointestinal kliniklerinde protozoan parazitler ve ’nın prevalansını ve dağılımını araştırdık. Güney Afrika’nın kırsal ve kentsel topluluklarında ve enterik parazitlerinin sıklığını araştırmak için kesitsel bir çalışma yapıldı. İshali olan (n=227) ve olmayan (n=257) hastaların Kasım 2013-Haziran 2015 tarihleri arasında dışkı örnekleri toplandı. DNA ekstrakte edildi ve bu protozoan parazitleri saptamak için tanısal bir Taqman qPCR tahlili kullanılarak, birkaç örnek Sanger dizilimi ile daha da doğrulandı. Toplanan 484 dışkı örneğinin %34’ü (166/484) spp. veya parazitleri için pozitifti ve örneklerin sadece %5’i her iki paraziti de içeriyordu (22/484). Her iki çalışma popülasyonunda da en yaygın parazitti (toplam %25) ve bunu (%19) izledi. Bu çalışma, hem hem de parazitlerinin Güney Afrika’daki ishal hastalığına katkıda bulunabileceğini ve kırsal topluluklarda daha yaygın olduğunu göstermiştir. Hem enfeksiyonun kaynağını belirlemek hem de hastalığın yükünü azaltmak için uygun müdahaleleri tasarlamak için gelecekteki çalışmalara ihtiyaç vardır.

Introduction: Human microsporidiosis represents an important and rapidly emerging opportunistic disease. The present study investigated the prevalence of microsporidia among HIV positive and HIV negative patients with or without diarrhoea in Vhembe and Mopani Districts in the Limpopo Province. Methodology: A total of 170 stool samples were collected from these patients and microsporidia species was detected using a Real-Time PCR targeting a conserved region of the small ribosomal subunit rRNA (SSU-rRNA) gene of Enterocytozoon bieneusi, Encephalitozoon intestinalis, Encephalitozoon hellem, and Encephalitozoon cuniculi. Results: Fifty six (32.9%) were positive for microsporidia. The prevalence was higher in HIV negative patients (36.6%) while 24.1% of patients who were HIV positive had microsporidia. Microsporidia was more common among patients aged between 1 and 10 years (52.6%). However among the HIV positive patients, microsporidia prevalence was higher among those that were not taking antiretrovirals (ARVs) compared to those who were on ARVs, (36.6%) and (24.1%), respectively. Microsporidia was also noted to be significantly associated with diarrheal and stomach pains; p = 0.02 and p = 0.048, respectively. Furthermore, microsporidia infections was more prevalent among patients who had animals at home (p = 0.037). Conclusions: Study has shown a high prevalence of microsporidia among patients attending primary health centers in the Mopani District for the first time. Prevalence of microsporidia was higher among HIV negative and HIV positive patients who were not on ARV treatment. Keeping animals in the household appeared to be a risk of getting infected with microsporidia. Further studies are needed to determine the genetic characteristics of these organisms in the study population.

Dengue virus (DENV) causes a spectrum of diseases ranging from asymptomatic, mild febrile to a life-threatening illness: dengue hemorrhagic fever. The main clinical symptom of dengue is fever, similar to that of malaria. The prevalence of dengue virus infection, alone or in association with other endemic infectious diseases in children in Cameroon is unknown. The aim of this study was to determine the prevalence of dengue, malaria and HIV in children presenting with fever and associated risk factors. Dengue overall prevalence was 20.2%, Malaria cases were 52.7% and HIV cases represented 12.6%. The prevalence of dengue-HIV co-infection was 6.0% and that of Malaria-dengue co-infection was 19.5%. Triple infection prevalence was 4.3%. Dengue virus infection is present in children and HIV-Dengue or Dengue- Malaria co-infections are common. Dengue peak prevalence was between August and October. Sex and age were not associated with dengue and dengue co-infections. However, malaria as well as HIV were significantly associated with dengue (P = 0.001 and 0.028 respectively). The diagnosis of dengue and Malaria should be carried out routinely for better management of fever.

Consumption of microbial-contaminated water can result in diarrheal illnesses and enteropathy with the heaviest impact upon children below the age of five. We aimed to provide a comprehensive analysis of water quality in a low-resource setting in Limpopo province, South Africa. Surveys were conducted in 405 households in rural communities of Limpopo province to determine their water-use practices, perceptions of water quality, and household water-treatment methods. Drinking water samples were tested from households for microbiological contamination. Water from potential natural sources were tested for physicochemical and microbiological quality in the dry and wet seasons. Most households had their primary water source piped into their yard or used an intermittent public tap. Approximately one third of caregivers perceived that they could get sick from drinking water. All natural water sources tested positive for fecal contamination at some point during each season. The treated municipal supply never tested positive for fecal contamination; however, the treated system does not reach all residents in the valley; furthermore, frequent shutdowns of the treatment systems and intermittent distribution make the treated water unreliable. The increased water quantity in the wet season correlates with increased treated water from municipal taps and a decrease in the average contaminant levels in household water. This research suggests that wet season increases in water quantity result in more treated water in the region and that is reflected in residents’ water-use practices.

Background Deoxyribonucleic acid (DNA) methylation is one of the epigenetic modifications that has gained a lot of interest as a factor influencing fetal programming and as a biomarker for adverse pregnancy and birth outcomes (APBOs). Epidemiological studies have demonstrated that DNA methylation can result in adverse pregnancy and birth outcomes (APBOs) including miscarriage, intrauterine growth restriction (IUGR), low birth weight (LBW), sepsis, and preterm birth (PTB), which may later result in diseases in adulthood. However, the mechanism by which DNA methylation influences these APBOs remains unclear. The systematic review will assess the association between global and gene-specific DNA methylation with adverse pregnancy outcomes. Method The Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) 2020 checklist will be followed when conducting this systematic review. To develop the search strategy the PI(E)COS (population, intervention/exposure, comparator/control, outcome, and study designs) framework will be followed. Thus far, the research team has retrieved 4721 from Cochrane Library, PubMed, Web of Sciences, and MEDLINE. Out of these, 584 studies have been screened for eligibility, and approximately 124 studies meet the inclusion criteria. Pending the search results identified from the grey literature. For identification of unpublished studies in journals indexed in electronic databases, Google Scholar will be used. I.M and A.S will separately extract data from the articles and screen them, if there are any disagreements between I.M and A.S, then the L.M will resolve them. The methodological quality and bias risk of the included studies will be evaluated using the Critical Appraisal Skill Programme CASP) checklist. I 2 and χ 2 alpha = 0.10 statistic will be used for assessing statistical heterogeneity between studies. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach will be used to assess and grade the overall quality of extracted data. Ethics and dissemination Ethical approval is not required. The systematic review will assess available literature on possible associations between DNA methylation with adverse pregnancy and birth outcomes (APBOs) including LBW, IUGR, miscarriage, sepsis, and PTB. The findings could help guide future research assessing DNA methylation and other APBOs. Systematic review registration PROSPERO CRCRD42022370647.