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15 result(s) for "Brogden, William A"
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Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the ~29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
Cocoon 2 programming : Web publishing with XML and Java
Thanks to the tireless efforts of open-source developers, Cocoon has quickly gained visibility as the preeminent XML-based Web publishing framework. Unfortunately, its documentation remains a significant shortcoming. If you're new to Cocoon, gaining a sense of exactly what you can do with it can be difficult, and actually getting started can be even harder. Cocoon 2 Programming: Web Publishing with XML and Java clearly explains the value of Cocoon and helps you build on your familiarity with XML and Java Servlets as you design, build, and implement a range of Cocoon applications. You'll begin by learning to control presentation for various platforms, both wired and wireless. Then you'll move on to Cocoon's capabilities for logic control and content management, using both sitemaps and XSP to create a site dynamically generated from a variety of data sources and types. As you'll see, Cocoon also supports powerful organizing techniques known as design patterns, and you'll master their use in both presentation and content generation. These are essential tools for the planning of your site; for after it's implemented, you'll command powerful techniques for site management and optimization. The Web's dependence on XML is growing rapidly--and with it the list of companies that have adopted Cocoon. Is Cocoon for you? Cocoon 2 Programming will guide you through your decision and far beyond, providing all the assistance you need to build a dynamic, XML-based site--and keep moving into the future.
Terra nullius: encountering the non-place
This practice-led Ph.D. study examines the potential conception of urban non-places through art photographic practice and writing (the text). The term “non-place(s)” (borrowed from Marc Augé, the French anthropologist) is used within this study as a deliberate provocation to the reader to re-engage with those transitional areas of land situated in the urban landscape that are viewed as interstitial - without a clear function or meaning compared to more valued spaces in the city, such as parks, and memorial sites. In general, these non-places are prohibited sites, which to some degree, adds to their anonymity in relation to the general public’s awareness. Most people (apart from a growing interest from urban psycho-geographers) avoid these sites, with their knowledge largely based upon the ubiquitous version promulgated by the media and potential developers of these “wasteland” / “brown field” sites (often associated with those areas of derelict land left abandoned since the 1970s post-industrial decline in England), which through such nomenclature, serves to under-value the unique qualities that this study seeks to present /reclaim. In this sense, one of the discursive aims of this study is to challenge the more expedient perception of such places by critically ‘erasing’ the prefix non, to reveal a place with meaning, that might be valued in a more imaginative way. In this sense, the practice acts as both a critical catalyst, and conduit, through which various disciplines are conflated in order to propose a different conception of non-places. A key aim within this text, is to emphasise the synergy between photographic practice and its equivalent in writing. Each embodies the other to form the practice. They are not separate elements within the Ph.D. submission. Although the leitmotiv of the study is located in the field of landscape representation, the text includes an eclectic range of allusions from within visual culture, to promote a broader academic debate between photography and the expanding fields of landscape studies, new cultural geography, anthropology, memory studies, new urbanism, and eco-criticism. The study proposes that through such an inter-disciplinary discourse mediated by photographic practice, a more idiosyncratic critical lens might emerge to challenge how we might conceive the contemporary Terra Nullius - the urban non-place(s). This text should be read in conjunction with viewing the Dossier (Volume 2): a visual record of the photographic archive that was produced during the period of the Ph.D. study. It should also be considered in relation to the works included in the final exhibition of photographs Terra Nullius: Encountering the Non-Place, which took place in The Corridor Gallery, School of Design, University of Leeds, in December 2010. A list of these works follows.
CHARACTERIZATION OF FRESHWATER AND ESTUARINE HUMIC ACIDS BY MOLECULAR WEIGHT DISTRIBUTION
In this work gel chromatography has been applied to the study of humic acids dissolved in natural waters using samples at natural concentration. Through the use of specially devised ultracentrifuge techniques for the analysis of polydisperse mixtures, it has been possible to verify the absence of significant absorption effects during gel chromatography.The molecular weights of humic acids in all Florida gulf coast river waters examined showed a continuous distribution over the range of resolution of the gel, approximately 5000 to 300. The amount of humic acids with molecular weights greater than 5000 was from 5 to 13 percent on an organic carbon basis. The majority of the remaining material had molecular weights between 5000 and 1000. In 5 out of 7 cases in which humic acid rich fresh water mixed with sea water in estuaries, there was evidence for the loss from solution of a substantial fraction of the humic acids with molecular weights greater than approximately 5000. This loss was also observed in 2 out of 3 laboratory experiments in which humic acid containing fresh water was mixed with seawater. The formation of a flocculent brown precipitate in the laboratory experiments indicates that the high molecular weight humic acids were lost by precipitation.The majority of the humic acids in fresh water were found to be stable in mixtures with seawater, and are expected to exert an important influence on estuarine and nearshore seawater chemistry.