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"Cheng, Yu-Ting"
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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
Ouzounov
et al
. report calcium imaging with three-photon microscopy in the mouse brain. The approach enabled noninvasive recording of activity with high spatial and temporal resolution from GCaMP6-labeled neurons located as deep as the hippocampus.
High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.
Journal Article
Hospital volume and outcomes of surgical repair in type A acute aortic dissection: A nationwide cohort study
Over the last decade, the number of patients treated with open repair for TAAAD in Taiwan has dramatically increased. This study aims to assess the hospital-volume relationship with surgical outcomes of type A acute aortic dissection (TAAAD) across hospitals in Taiwan.
Using the Taiwan National Health Insurance (NHI) Research Database (NHIRD), we include only the patients who underwent first open repair for TAAAD from 01/01/2005, to 31/12/2020, in Taiwan. A total of 8,059 patients in 77 hospitals were eligible for the analysis. Hospitals were categorized based on their 16-year cumulative volume of TAAAD open repair surgeries, and patients were grouped into quartiles accordingly.
Ascending aortic replacement (55.7%) and partial/total arch replacement (38.8%) were the most common methods of open aortic repair. In-hospital mortality was 22% and decreased from 28% in 2005 to 20% in 2020. Greater volume (per 5 annual surgeries) was associated with lower risks of in-hospital mortality (odd ratio 0.90, 95% confidence interval [CI] 0.87-0.92) and mortality after discharge (hazard ratio 0.97, 95% CI 0.95-0.99).
Operative volume inversely correlates to in-hospital mortality and postoperative complications. The volume-outcome effect extends after discharge and reflects better long-term survival. Hospital referral to high-volume centers should be considered in patients needing complex open repair for TAAAD.
Journal Article
Evaluation of commercially available class A water-based foam concentrates for swine depopulation
Currently, the swine industry is lacking an efficient method for large-scale emergency depopulation. Class A water-based foam (WBF) has been demonstrated as a viable option for large-scale depopulation of pigs in all stages of development. However, these studies exclusively used the PHOS-CHEK WD881 (WD881) Class A foam concentrate based on previously demonstrated efficacy for depopulation. This study investigated the suitability of 15 other commercially available WBF concentrates for depopulation based on foaming performance, physiological effects, and efficacy. The performance of each product was evaluated and compared to WD881 at 0.5, 1.0 and 3.0% water-foam concentrations for low- and high-pressure pump systems. The time to fill an 11.5 m 3 construction container and decay rate over a 10-minute dwell time were assessed for each WBF. Top-performing foams were further evaluated for behavior and short-term physiological changes and gross lesions during a 15-minute exposure test on piglets. Finally, the top-performing foams were tested for their suitability to depopulate adult swine during large-scale field conditions. Subcutaneous dataloggers recorded swine activity which was used to estimate the time to cessation of movement (COM), an approximate analog for loss of consciousness. Four WBF concentrates (FireIce Polar EcoFoam, Buckeye Platinum, National Foams Knockdown and BioFor N) were shortlisted based on performance at 1% concentration. These products had a mean (±SD) fill time of 62.4s (± 14.9) and decay rate of 0.5 (± 0.66) cm/min compared to WD881 with 50.0s (± 3.5) and 0.2 (± 0.1) cm/min, respectively. No differences between treatment groups were observed during the exposure testing and subsequent necropsy. For the large-scale field trials, the mean (±SE) time to COM was 151.5 s (±10.5). All foams achieved 100% mortality of swine. This study identified four additional WBFs suitable for swine depopulation which are commercially available on the U.S market. These additional WBF options may facilitate large-scale swine depopulation during widespread infectious disease outbreaks by mitigating potential bottlenecks resulting from product availability.
Journal Article
Application of Support Vector Machine (SVM) in the Sentiment Analysis of Twitter DataSet
At present, in the mainstream sentiment analysis methods represented by the Support Vector Machine, the vocabulary and the latent semantic information involved in the text are not well considered, and sentiment analysis of text is dependent overly on the statistics of sentiment words. Thus, a Fisher kernel function based on Probabilistic Latent Semantic Analysis is proposed in this paper for sentiment analysis by Support Vector Machine. The Fisher kernel function based on the model is derived from the Probabilistic Latent Semantic Analysis model. By means of this method, latent semantic information involving the probability characteristics can be used as the classification characteristics, along with the improvement of the effect of classification for support vector machine, and the problem of ignoring the latent semantic characteristics in text sentiment analysis can be addressed. The results show that the effect of the method proposed in this paper, compared with the comparison method, is obviously improved.
Journal Article
Assessment of three large-scale depopulation methods for swine
The threat of foreign animal disease outbreaks to U.S. swine herds warrants effective and readily available depopulation methods. Current American Veterinary Medical Association-recommendations using preferred physical methods for swine depopulation are unsuitable for large commercial swine herds. Our objectives were to assess and compare the efficacy and performance of three suggested large-scale depopulation methods: 1) medium-expansion water-based foam, 2) prototype high-expansion nitrogen foam and, 3) carbon dioxide gas for finisher pigs under field conditions. Out of 793 finisher pigs included in the study, 84 were implanted with bio-loggers recording electrocardiogram and pig movement data. Aversive pig behaviors were collected manually on a group level for each depopulation method. A subsample of pigs from each method were examined post-mortem for lesions and compared to a reference group of nine pigs euthanized with pentobarbital. Depopulation method assessments included container fill time, the number of aversive pig behaviors observed during depopulation, overall pig movement intensity, time to cessation of movement, time to and cause of cardiac arrest, and respiratory lesions. No difference in fill times between water-based foam and nitrogen foam was observed. The total number of aversive swine behaviors was higher for carbon-dioxide compared to both foam methodologies ( P < 0.01). The total pig activity was higher in water-based foam compared to nitrogen foam ( P = 0.02) and carbon-dioxide methods ( P = 0.01). The mean time to cessation of movement was significantly shorter for water-based foam and nitrogen foam compared to carbon-dioxide ( P < 0.01). No differences in cardiac activity were observed. Water-based foam pigs had increased odds of distal trachea occlusions compared to other methods. All depopulation methods demonstrated high efficacy with a 100% mortality rate. The results from this study support large-scale water-based foam, nitrogen foam and carbon dioxide as viable AVMA depopulation guideline candidates for swine.
Journal Article
Sex-related differences on the risks of in-hospital and late outcomes after acute aortic dissection: A nationwide population-based cohort study
The aim of this study is to evaluate the sex-related differences on the risks of perioperative and late outcomes for adult acute aortic dissection (AAD) patients following surgical management.
By using Taiwan National Health Insurance Research Database, totally 1,410 female and 3,432 male patients were identified to first-ever receive type A AAD open surgery or type B AAD stenting treatment from 2004 to 2013. We assessed the sex-related difference on outcomes, including in-hospital mortality, all-cause mortality, aortic death, redo aortic surgery, ischemic stroke, and depression during the follow-up period. The analysis was done separately for type A and type B surgeries.
On average, female patients diagnosed with AAD were older than males. There was no significant sex difference of in-hospital mortality or all-cause mortality for both type A open and type B stent surgeries. The risk of redo aortic surgery was significantly greater in males than females (7.8% vs. 4%; unadjusted subdistribution hazard ratio [SHR] 0.51, 95% CI 0.38-0.69) for type A open surgery, but not for type B stent surgery. Noticeably, the risk of newly-diagnosed depression was significantly greater in females than males (8% vs. 5.1%; unadjusted SHR 1.6, 95% CI 1.24-2.06) for type A open surgery, but not for type B stent surgery.
No significant sex-related difference was found for the in-hospital mortality or accumulative all-cause mortality. However, there were more redo aortic surgeries for males and more postoperative depression for females in type A AAD population.
Journal Article
Use of fluoroquinolones and the risk of aortic and mitral regurgitation: A nationwide case-crossover study
Recently, there have been conflicting results reporting an increased risk of AR or MR associated with oral fluoroquinolones (FQs).This study investigated whether the use of FQs increases the risk of mitral regurgitation (MR) or aortic regurgitation (AR).
A retrospective cohort study was conducted by using the Taiwan National Health Insurance research database. A unidirectional case-crossover design without selecting controls from an external population was adopted in this study. A total of 26,650 adult patients with new onset of AR or MR between January 1, 2000, and December 31, 2012, were identified. The risk of outcomes was compared between the hazard period and one of the randomly selected referent periods of the same individuals.
Before exclusion of pneumonia diagnosed within 2 months before the index date, patients who took FQs had a significantly greater risk of AR or MR (adjusted odds ratio [aOR] 1.51, 95% confidence interval [CI] 1.30-1.77), any AR (combined AR and MR) (aOR 1.50, 95% CI 1.10-2.04), and any MR (combined AR and MR) (aOR 1.37, 95% CI 1.16-1.62). After exclusion of pneumonia, FQs exposure remained significantly associated with a greater risk of MR (aOR 1.38, 95% CI 1.17-1.62) and any MR (aOR 1.25, 95% CI 1.05-1.48).
The findings suggested that patients treated with FQs could be warned about the potential risk for MR even after considering the possibility of protopathic bias. Reducing unnecessary FQs prescriptions may be considered to reduce the risk of valvular heart disease.
Journal Article
Balloon-expandable versus self-expanding transcatheter aortic valve replacement for bioprosthetic dysfunction: A systematic review and meta-analysis
Transcatheter aortic valve-in-valve (VIV) procedure is a safe alternative to conventional reoperation for bioprosthetic dysfunction. Balloon-expandable valve (BEV) and self-expanding valve (SEV) are the 2 major types of devices used. Evidence regarding the comparison of the 2 valves remains scarce. A systematic review and meta-analysis was conducted to compare the outcomes of BEV and SEV in transcatheter VIV for aortic bioprostheses dysfunction. A computerized search of Medline, PubMed, Embase, and Cochrane databases was performed. English-language journal articles reporting SEV or BEV outcomes of at least 10 patients were included. In total, 27 studies were included, with 2,269 and 1,671 patients in the BEV and SEV groups, respectively. Rates of 30-day mortality and stroke did not differ significantly between the 2 groups. However, BEV was associated with significantly lower rates of postprocedural permanent pacemaker implantation (3.8% vs. 12%; P < 0.001). Regarding echocardiographic parameters, SEV was associated with larger postprocedural effective orifice area at 30 days (1.53 cm.sup.2 vs. 1.23 cm.sup.2 ; P < 0.001) and 1 year (1.55 cm.sup.2 vs. 1.22 cm.sup.2 ; P < 0.001). For patients who underwent transcatheter aortic VIV, SEV was associated with larger postprocedural effective orifice area but higher rates of permanent pacemaker implantation. These findings provide valuable information for optimizing device selection for transcatheter aortic VIV.
Journal Article
Zoledronic acid inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways
It is well known that extensive osteoclast formation plays a key role in osteoporosis in post-menopausal women and the elderly. The suppression of extensive osteoclastogenesis and bone resorption may be an effective preventive strategy for osteoporosis. Zoledronic acid (ZOL) has been indicated to play an essential role in regulating bone mineral density and has already been used in large clinical trials. However, the effects of ZOL on osteoclastogenesis remain to be fully elucidated. Therefore, the present study aimed to determine the effects of ZOL on osteoclastogenesis, and to explore the corresponding signalling pathways. By using a cell viability assay, as well as in vitro osteoclastogenesis, immunofluorescence and resorption pit assays, we demonstrated that ZOL (0.1-5 µM) suppressed receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation and bone resorptive activity. Furthermore, western blot analysis and reverse transcription-quantitative PCR indicated that ZOL inhibited the RANKL-induced activation of NF-κB and the phosphorylation of JNK in RAW264.7 cells, and subsequently decreased the expression of osteoclastogenesis-associated genes, including calcitonin receptor, tartrate-resistant acid phosphatase and dendritic cell-specific transmembrane protein. ZOL inhibited osteoclast formation and resorption in vitro by specifically suppressing NF-κB and JNK signalling. On the whole, the findings of this study indicate that ZOL may serve as a potential agent for the treatment of osteoclast-associated diseases, including osteoporosis.
Journal Article