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The genus Ficus of the family Moraceae, is one of the largest genera of angiosperms, with diverse pharmaceutical applications and biological activities. The traditional approaches based on the morphological traits have been frequently implemented for taxonomical identification of the different taxa of Ficus , however, encompassing these features are quite laborious, due to the dependence of these phenotypic traits on the environmental conditions. So, authenticating the taxonomical identity of the Ficus taxa with molecular barcoding and metabolic profiling, as relatively stable traits, could be a relevant approach for confirming the traditional phenotypic traits of this genus. Nine species of the genus Ficus namely F. amplissima Sm., F. benjamina L. F. binnendijkii , F. drupacea var. pubescens , F. elastica Roxb., F. microcarpa L., F. religiosa L., F. tinctoria subsp. gibbosa and F. virens var. sublancelata in Egypt, were selected for this study. From the anatomical features, three species of subsection Urostigma, F. religiosa , F. virens var. sublanceolata have cystoliths on the abaxial layer, whereas in F. amplissima it was on the adaxial layer. The UPGMA dendrogram of the studied Ficus taxa has been generated from the 21 anatomical characters, categorized the studied taxa into two clusters (I and II) of average distance ~ 3.5, each cluster has been further divided into subclusters I and II. The sub-cluster I includes F. religiosa , F. virens var. sublanceolata and F. tinctoria subsp. gibbosa were grouped together to subsection Urostigma, while the sub-cluster II of the cluster I includes F. benjamina and F. amplissima . From the DNA barcoding analysis, three clusters I, II and III were emerged, the cluster I includes F. benjamina , F. binnendjikee , and F. amplissima . The cluster II, F. virens var. sublanceolata and F. religiosa that belong to subsection Urostigma, while, the cluster III includes F. elastica and F. drupacea var. pubescens , F. microcarpa that belongs to subsection Conosycea. From the metabolic profiling of Ficus species, the major compounds; H-cycloprop-azulen-7-ol, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, 2-(9-octadecenyloxy), pentadecanoic acid, phytol, sitosterol and 9,12-octadecadienoic acid were the common among the taxa, with an obvious fluctuation, that could be a chemotaxonomic markers for these species of Ficus . Based on the metabolic profiling, two distinct clusters I and II were evolved, the cluster I involve F. elastica , F. benjamina , F. drupacea var. pubescens , F. amplissima , while, the cluster II had F. tinctoria subsp. gibbosa and F. religiosa . The fluctuation on the metabolites of the tested Ficus species could be a metabolic fingerprint for each species. So, the delamination of the tested plants based on their anatomical traits was typically matched to the separation based on the ITS sequence analysis.

The ornamental palms represent a diverse species in the national botanical gardens, and roadsides; however, the accurate identification of the palm trees (Arecaceae) is a problematic due to the numerous overlapped morphological traits, especially with the environmental conditions. So, the objective of this study was to implement the different morphological traits, especially based on the pyrene morphology, with the molecular barcoding markers of the plastid rbcL on delineation and revising the taxonomical identification of the most common Palm trees in Egypt in addition to their pharmacological and Ethnobotanical applications. An obvious variation on the surface of pyrenes among the studied Palm taxa ranged from ovoid to globose or discoid, with brown to pale brown, was recorded. The pyrene's fruit dimensions were ranged with S. yaba Becc. (5.65 × 6.85 mm), Washingtonia robusta (7.19 × 4.4 mm) and Sabal palmetto (Walter) Lodd. ex Schult. & Schult.f. (7.24 × 9.58 mm), while Syagrus romanzoffiana (Cham.) Glassman is (19.8 × 12.15 mm). The color of the pyrene of W. robusta , S. romanzoffiana , and Livistona decora (W.Bull) Dowe was brown, while was dark brown in Butia capitata (Mart.) Becc . Sabal yapa and S. palmetto. The SEM analysis of the pyrene surface microsculpture, the studied taxa of S. palmetto, S. yaba, Livistona, Brahea, and Sabal could be easily delimited at the generic level. The taxonomical identification of plant taxa based on their morphological characteristics, such as color, surface smoothness, and geometric shapes, was confirmed based on their molecular barcoding. The Principal Component Analysis (PCA) scatter plot based on the morphological traits distinguishes the taxa of tribe Cocoeae, subfamily Arecoideae and taxa of tribe Corypheae, subfamily Coryphoideae. From the UPGMA dendrogram based on the micromorphological characteristics, the studied taxa were grouped into two major clusters (I, II), the cluster I includes S. palmatto, S, yaba and W. robusta which belongs to subtribe Sabalinae, tribe corypheae, while cluster II includes L. decora, L. chinensis, (Jacq.) R.Br. ex Mart.and B. armata which belongs to subtribe Livistoninae and tribe Corypheae. Thus, the classification of the experimental plants based on the morphological traits of pyrene fruit microsculpturing was closely matched with the molecular barcoding based on the rbcL sequences.

Background The resilience of plants against environmental challenges, particularly salinity and dehydration, is crucial for global food security. This study delves into the intricate interaction between NaCl-induced salinity and Azolla aqueous extract (AAE). In a pot trial, wheat kernels were primed with deionized water or 0.1% AAE for 21 h. Seedlings underwent various treatments; tap water, 250 mM NaCl, AAE priming and spray, and combined AAE with NaCl treatments. Seedlings were analyzed for ionic balance, secondary metabolism, antioxidant efficacy, and molecular response to experimental treatments. Results GC-MS analysis of AAE revealed key components like γ-aminobutyric acid and benzenedicarboxylic acid. Exposure to 250 mM NaCl significantly reduced N, P, Ca, K, and the K/Na ratio, while increases in Mg and Na. Also, salinity significantly decreased TAC, DPPH activity, and AsA levels while increasing GB in wheat seedlings. Additionally, salinity increased flavonoids, saponins, and anthocyanins but non-significantly decreased phenols. qRT-PCR analysis revealed upregulation of DRF1, CBF3, HQT, CHS, and FLS genes and downregulation of CBF4 and CHI genes by salinity. AAE treatments, alone or combined with salt stress, mitigated Na accumulation (31.50 and 32.87% compared to stressed seedlings), improved N and P levels, alleviated Mg, K/Na, and GB imbalances, and enhanced antioxidant potentials. Combined AAE and NaCl treatments effectually restored antioxidant potentials and regulated secondary metabolites and gene expressions, sustaining enhancement of ionic equilibrium, antioxidant defenses, and molecular responses in salt-stressed wheat. Conclusions Overall, AAE can be exploited as a green approach for sustaining normal metabolism and gene expression of wheat seedlings in saline soils.

The ornamental palms represent a diverse species in the national botanical gardens, and roadsides; however, the accurate identification of the palm trees (Arecaceae) is a problematic due to the numerous overlapped morphological traits, especially with the environmental conditions. So, the objective of this study was to implement the different morphological traits, especially based on the pyrene morphology, with the molecular barcoding markers of the plastid rbcL on delineation and revising the taxonomical identification of the most common Palm trees in Egypt in addition to their pharmacological and Ethnobotanical applications. An obvious variation on the surface of pyrenes among the studied Palm taxa ranged from ovoid to globose or discoid, with brown to pale brown, was recorded. The pyrene's fruit dimensions were ranged with S. yaba Becc. (5.65 × 6.85 mm), Washingtonia robusta (7.19 × 4.4 mm) and Sabal palmetto (Walter) Lodd. ex Schult. & Schult.f. (7.24 × 9.58 mm), while Syagrus romanzoffiana (Cham.) Glassman is (19.8 × 12.15 mm). The color of the pyrene of W. robusta, S. romanzoffiana, and Livistona decora (W.Bull) Dowe was brown, while was dark brown in Butia capitata (Mart.) Becc. Sabal yapa and S. palmetto. The SEM analysis of the pyrene surface microsculpture, the studied taxa of S. palmetto, S. yaba, Livistona, Brahea, and Sabal could be easily delimited at the generic level. The taxonomical identification of plant taxa based on their morphological characteristics, such as color, surface smoothness, and geometric shapes, was confirmed based on their molecular barcoding. The Principal Component Analysis (PCA) scatter plot based on the morphological traits distinguishes the taxa of tribe Cocoeae, subfamily Arecoideae and taxa of tribe Corypheae, subfamily Coryphoideae. From the UPGMA dendrogram based on the micromorphological characteristics, the studied taxa were grouped into two major clusters (I, II), the cluster I includes S. palmatto, S, yaba and W. robusta which belongs to subtribe Sabalinae, tribe corypheae, while cluster II includes L. decora, L. chinensis, (Jacq.) R.Br. ex Mart.and B. armata which belongs to subtribe Livistoninae and tribe Corypheae. Thus, the classification of the experimental plants based on the morphological traits of pyrene fruit microsculpturing was closely matched with the molecular barcoding based on the rbcL sequences.