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Biofilms contribute to bacterial infection and drug resistance and are a serious threat to global human health. Antibacterial nanomaterials have attracted considerable attention, but the inhibition of biofilms remains a major challenge. Herein, we propose a nanohole-boosted electron transport (NBET) antibiofilm concept. Unlike known antibacterial mechanisms (e.g., reactive oxygen species production and cell membrane damage), nanoholes with atomic vacancies and biofilms serve as electronic donors and receptors, respectively, and thus boost the high electron transport capacity between nanomaterials and biofilms. Electron transport effectively destroys the critical components (proteins, intercellularly adhered polysaccharides and extracellular DNA) of biofilms, and the nanoholes also significantly downregulate the expression of genes related to biofilm formation. The anti-infection capacity is thoroughly verified both in vitro (human cells) and in vivo (rat ocular and mouse intestinal infection models), and the nanohole-enabled nanomaterials are found to be highly biocompatible. Importantly, compared with typical antibiotics, nanomaterials are nonresistant and thereby exhibit high potential for use in various applications. As a proof-of-principle demonstration, these findings hold promise for the use of NBET in treatments for pathogenic bacterial infection and antibiotic drug resistance. Nanomaterials have attracted attention as antibacterial agents and have several modes of action. Here, the authors report on 2D transition metal disulphide nanosheets with hole boosted electron donation/withdrawal for enhanced antibacterial and biofilm activity caused by electron damage.

Regulated deficit irrigation (RDI) is an important water-saving strategy in arid regions. To quantify the effects of RDI on foxtail millet yield and water use efficiency and determine an optimal RDI strategy, a three-year field trial was carried out over dry, normal, and wet rainfall years in the Loess Plateau. Full irrigation throughout the whole growth period served as the control, whereas mild, moderate, and severe deficit irrigation treatments were conducted at the jointing–booting stage, heading–flowering stage, and across the whole growing period, respectively. The results indicate that the effects of RDI on foxtail millet yield varied with crop growth stage and deficit severity. During the heading–flowering stage, mild RDI showed statistically similar grain yield and WUE relative to those under full irrigation. In normal and wet years, moderate and severe RDI had no statistically significant effects on grain yield and WUE. Additionally, moderate and severe RDI significantly improved irrigation water use efficiency by 19.94–28.50% and 34.35–47.72%, respectively. The primary reason is that RDI at this stage maintained root development and led to only limited suppression of plant growth. In contrast, moderate and severe RDI at the jointing–booting stage or throughout the whole growth period significantly inhibited root establishment and plant development, reduced dry matter accumulation, and consequently led to substantial yield losses. The inhibitory effect became more pronounced with increasing deficit severity. Specifically, severe RDI at the jointing–booting stage and throughout the entire growth period significantly reduced yield by 19.35–54.98% and 31.47–100%, respectively. Furthermore, to identify the optimal RDI regime adaptable to variable rainfall years, a multi-model comprehensive evaluation system based on yield and WUE was established by integrating three individual evaluation models, including the membership function method, TOPSIS, and grey relational analysis, with the Fuzzy–Borda combined evaluation model. The result showed that the heading–flowering stage is the critical period for implementing RDI in foxtail millet in the Loess Plateau. Mild RDI during this stage is preferred because it maintains stable yield and WUE while substantially reducing irrigation amount over various rainfall years. Additionally, moderate and severe RDI can also maintain stable yield while significantly improving irrigation water use efficiency in normal and wet years.

Background Hull colour is an important morphological marker for selection in seed production of foxtail millet. However, the molecular mechanisms underlying hull colour variation remain unknown. Results An F 7 recombinant inbred line (RIL) population containing 215 lines derived from Hongjiugu × Yugu18 was used to analyze inheritance and detect the quantitative trait loci (QTL) for four hull colour traits using major gene plus polygene mixed inheritance analysis and composite interval mapping (CIM) in four environments. Genetic analysis revealed that the hull colour L * value (HCL * ) was controlled by two major genes plus additive polygenes, the hull colour a * value (HCa * ) was controlled by three major genes, the hull colour b * value (HCb*) was controlled by two major genes plus polygenes, and the hull colour C * value (HCC*) was controlled by four major genes. A high-density genetic linkage map covering 1227.383 cM of the foxtail millet genome, with an average interval of 0.879 cM between adjacent bin markers, was constructed using 1420 bin markers. Based on the genetic linkage map and the phenotypic data, a total of 39 QTL were detected for these four hull colour traits across four environments, each explaining 1.50%–49.20% of the phenotypic variation. Of these, six environmentally stable major QTL were co-localized to regions on chromosomes 1 and 9, playing a major role in hull colour. There were 556 annotated genes within the two QTL regions. Based on the functions of homologous genes in Arabidopsis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) gene annotations, five genes were predicted as candidate genes for further studies. Conclusions This is the first study to use an inheritance model and QTL mapping to determine the genetic mechanisms of hull colour trait in foxtail millet. We identified six major environmentally stable QTL and predicted five potential candidate genes to be associated with hull colour. These results advance the current understanding of the genetic mechanisms underlying hull colour traits in foxtail millet and provide additional resources for application in genomics-assisted breeding and potential isolation and functional characterization of the candidate genes.

Improving cropping systems together with suitable agronomic management practices can maintain dry farming productivity and reduce water competition with low N inputs. The objective of the study was to determine the photosynthetic and yield responses of maize and peanut under six treatments: sole maize, sole peanut, maize–peanut intercropping, maize–peanut rotation–intercropping, 20% and 40% N reductions for maize in the maize–peanut rotation–intercropping. Maize–peanut intercropping had no land-use advantage. Intercropped peanut is limited in carboxylation rates and electron transport rate (ETR), leading to a decrease in hundred-grain weight (HGW) and an increase in blighted pods number per plant (N BP ). Intercropped peanut adapts to light stress by decreasing light saturation point (I sat ) and light compensation point (I comp ) and increasing the electron transport efficiency. Intercropped maize showed an increase in maximum photosynthetic rate (Pn max ) and I comp due to a combination of improved intercellular CO 2 concentration, carboxylation rates, PSII photochemical quantum efficiency, and ETR. Compare to maize–peanut intercropping, maize–peanut rotation–intercropping alleviated the continuous crop barriers of intercropped border row peanut by improving carboxylation rates, electron transport efficiency and decreasing I sat , thereby increasing its HGW and N BP . More importantly, the land equivalent ratio of maize–peanut rotation–intercropping in the second and third planting years were 1.05 and 1.07, respectively, showing obvious land use advantages. A 20% N reduction for maize in maize–peanut rotation–intercropping does not affect photosynthetic character and yield for intercropped crops. However, a 40% N reduction decreased significantly the carboxylation rates, ETR, I comp and Pn max of intercropped maize, thereby reducing in a 14.83% HGW and 5.75% lower grain number per spike, and making land-use efficiency negative.

Foxtail millet (Setaria italica L.) is an important cereal for managing future water scarcity and ensuring food security, due to its strong drought and salt stress resistance owing to its developed root system. However, the molecular responses of foxtail millet leaves to salt stress are largely unknown. In this study, seeds of 104 foxtail millet accessions were subjected to 0.17 mol·L−1 NaCl stress during germination, and various germination-related parameters were analyzed to derive 5 salt-sensitive accessions and 13 salt-tolerant accessions. Hong Gu 2000 and Pu Huang Yu were the most salt-tolerant and salt-sensitive accessions, respectively. To determine the mechanism of the salt stress response, transcriptomic differences between the control and salt-treated groups were investigated. We obtained 2019 and 736 differentially expressed genes under salt stress in the salt-sensitive and salt-tolerant accessions, respectively. The transcription factor families bHLH, WRKY, AP2/ERF, and MYB-MYC were found to play critical roles in foxtail millet’s response to salt stress. Additionally, the down-regulation of ribosomal protein-related genes causes stunted growth in the salt-sensitive accessions. The salt-tolerant accession alleviates salt stress by increasing energy production. Our findings provide novel insights into the molecular mechanism of foxtail millet’s response to salt stress.

It is difficult to design the engine intake manifold in normal method because of the complex structure, and the real situation of fluid movement in the manifold cannot be obtained through the steady flow test, so the optimized design is proposed about the engine intake manifold based on reverse engineering. A3D model of the intake manifold is build according to the CATIA software; fluid mathematical is set up by computational fluid dynamics method. The 3D geometry model of the intake manifold is grid with the Gambit software and the divided grid data is imported into the Fluent. These are performed about the simulation of the fluid pressure field, velocity field, flow field, and local flow field in the manifold. Key parameters design of the intake manifold relies on the results. It proves that the reverse technology is reasonable for the intake manifold design through the theory and simulation analysis; it can ensure sufficient air intake and reduce the intake resistance and energy loss of the air, and can improve the air intake uniformity.

Variation in abiotic conditions along altitudinal gradients may sort plant species from regional species pools according to their seed mass. With increasing elevation, seed mass is expected to be either larger for its advantage during seedling establishment in stressful high-elevation environments ('stress-tolerance' mechanism), or smaller owing to energy constraints. Using a large trait database involving 1355 species from the northeastern verge of the Tibetan Plateau, we found that, overall, these two opposing mechanisms balanced out one another, resulting in non-significant seed mass–elevation relationship across all species after controlling for phylogeny. At the same time, we found that the influence of energy constraints on seed mass was indirect and mediated by the variation in plant height. Moreover, our results revealed a mass-dependent seed mass variation along elevation gradients: with increasing elevation small seeds tended to increase (supporting stress-tolerance mechanism) but large seeds tended to decrease (supporting energy-constraints mechanism). Finally, the seed mass–elevation relationships were significantly different among species with different life forms or different dispersal modes, but statistically similar for anemophilous and entomophilous species. This implies that life-history cycle, resource allocation pattern and availability of dispersals agents, rather than pollination efficiency, can affect the responses of seed mass to elevation. Together our results suggest that a comprehensive perspective is necessary when interpreting geographic distribution of even a single trait.

Since lithium-ion batteries have been put into use, the recession of work cycle efficiency at low temperatures has received widespread attention. In this paper, we investigated the effect of low temperature (−5°C, 0°C, 5°C) environments on the performance of lithium-ion batteries, which are well-known for their excellent discharge performance, cycle life, and safety. However, lithium-ion batteries exhibit significant capacity degradation at low temperatures, especially at 0°C, losing availability after only 10 cycles. Therefore, we conducted cycle degradation tests at 1C discharge and 100% charge-discharge conditions. In this paper, we analyzed the surface morphology, structure, and elemental composition of the positive and negative electrodes before and after the aging process by disassembling them into half-cells and using three physical characterization techniques: scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). Our study identifies the temperature range for accelerated aging, which provides guidance for the optimal use of lithium-ion batteries in low temperature environments, thus contributing to the rational utilization and enhanced the performance of lithium-ion batteries at low temperatures.

Key message The genetic architecture of 19 growth and development and yield-related traits was dissected by a GWAS in multi-environment of a diverse population consisting of 301 broomcorn millet accessions. Broomcorn millet ( Panicum miliaceum L.) is an ideal replant crop due to its advantages of drought resistance, short growth period, and strong adaptability. Understanding the genetic basis of agronomic traits is crucial for improving the efficiency of molecular breeding in broomcorn millet. In this study, a natural population consisting of 301 worldwide broomcorn millet accessions was genotyped using 208,169 high-quality single-nucleotide polymorphisms (SNPs). Nineteen agronomic traits, including growth period, plant architecture, and yield-related traits, were investigated across ten environments. Based on genomic and phenotypic data, a genome-wide association study (GWAS) was performed to identify significant marker–trait associations (MTAs) affecting these traits. Using the linear mixed model, 662 significant MTAs were identified for 19 traits, with 56 stable MTAs repeatedly identified over two environments. Among these stable SNPs, 40 were located in genomic regions where no relevant loci had been reported previously. Notably, superior alleles of four significant SNPs chr1_2925777, chr7_157147, chr4_3971792, and chr5_2126999 associated with panicle length (PL) and the whole growth period (GP) exhibited significantly higher phenotypic levels. Ultimately, 174 genes within the flanking regions of these four significant SNPs were annotated. Among these, six genes were identified as candidate genes associated with PL and GP. Further haplotype analysis identified seven haplotypes for longmi011379 and longmi011388 . Phenotypic assessment of major haplotypes revealed significant differences between Hap1 and Hap2. These results provide a valuable resource for understanding the genetic mechanisms of growth and development and yield-related traits and developing marker-assisted selection breeding in broomcorn millet.

Aims The objective of this study was to propose a maize–peanut intercropping system for traditional sole maize to increase economic benefits and decrease environmental pollution for dryland farming. Methods The treatments comprised sole maize, sole peanut, maize–peanut intercropping, maize–peanut intercropping–rotation (rotation of the maize and peanut planting strips every year), and maize–peanut intercropping–rotation with 20% and 40% N reduction in maize planting strip. Soil water and total N stock, photosynthetic traits, yield, greenhouse gas emissions, economic income, and interspecific relationships were evaluated. Results Intercropped maize absorbed more moisture but lost more N than sole maize. Maize–peanut intercropping had higher economic benefits and less N 2 O emissions than sole maize, but had no intercropped advantage on land equivalent ratio. In addition, intercropping increased the continuous cropping barriers of peanuts than a sole peanut. Rotation of the planting strips in intercropping increased the peanut yield and economic benefits compared with not rotating the planting strips, and the economic benefits reached the highest level in the third planting year. A 20% N reduction in maize planting strips can decrease the global warming potential without affecting economic benefits. A 40% N reduction in maize planting strips could reduce soil N stock, maize yield, water use efficiency, N use efficiency, land equivalent ratio, and income. Conclusions A maize–peanut intercropping–rotation system is recommended for dryland farming since it promoted efficient use of N and economic benefits and decreased N 2 O emission. The proper N reduction in maize planting does not lead to N stress but decreased global warming potential.